Detailed information of TA system TA025352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 705464..706116
• Replicon: chromosome
• Accession: NC_015635
• Organism: Microlunatus phosphovorus NM-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5XKW8
• Locus tag: MLP_RS03085
• Protein ID: WP_013861545.1
• Coordinates: 705715..706116 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5XKW7
• Locus tag: MLP_RS03080
• Protein ID: WP_013861544.1
• Coordinates: 705464..705718 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLP_RS28470	700901..701488	+	588	Protein_609	LemA family protein	-
MLP_RS03060	702115..703056	+	942	WP_013861540.1	aldo/keto reductase	-
MLP_RS03065	703122..704033	+	912	WP_041789664.1	DUF2510 domain-containing protein	-
MLP_RS03070	704047..704700	-	654	WP_172641536.1	VTT domain-containing protein	-
MLP_RS03075	704789..705421	+	633	WP_197536561.1	rRNA methyltransferase	-
MLP_RS03080	705464..705718	+	255	WP_013861544.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MLP_RS03085	705715..706116	+	402	WP_013861545.1	type II toxin-antitoxin system VapC family toxin	Toxin
MLP_RS27825	706259..706414	-	156	WP_156821007.1	hypothetical protein	-
MLP_RS03090	706428..707738	-	1311	WP_013861546.1	acyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14313.32 Da
• Isoelectric Point: 5.7193

>T25352 WP_013861545.1 NC_015635:705715-706116 [Microlunatus phosphovorus NM-1]
MTVVVDTSAVLAVLLGEPDASRYADALSRNIGDLHMSAVSLVETRIVLESRKGPDAAADLETLIARVSIRVEAVTADQAD
RAWGAWRRFGKGCHPARLNLGDCFSYALVRALRGNLLYKGDDFSQTDVPALLG

Nucleotide

• Length: 402 bp

>T25352 NC_015635:705715-706116 [Microlunatus phosphovorus NM-1]
ATGACGGTCGTCGTCGACACATCGGCAGTGCTCGCCGTTCTGCTCGGTGAGCCCGACGCCTCGCGGTATGCCGACGCGCT
GTCCCGCAACATCGGGGACCTGCATATGAGTGCGGTCTCGCTCGTGGAGACACGCATCGTGCTGGAGTCTCGCAAGGGGC
CCGATGCGGCTGCCGACTTGGAGACTCTCATTGCCAGGGTGTCCATTCGGGTGGAGGCCGTGACGGCTGACCAGGCGGAC
CGTGCCTGGGGGGCGTGGCGGCGATTCGGGAAGGGTTGCCACCCGGCACGCCTCAACCTCGGTGATTGCTTCTCGTACGC
GCTCGTGCGGGCGTTGCGCGGCAACCTTCTTTACAAGGGCGATGACTTCTCTCAGACGGATGTGCCCGCACTGCTGGGTT
GA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9131.17 Da
• Isoelectric Point: 4.5642

>AT25352 WP_013861544.1 NC_015635:705464-705718 [Microlunatus phosphovorus NM-1]
MALNIKDPETDRLARELAAATGESITVAARRAIEERLERVRARADAGSASVDLGDLIERARSRPDLDVRSAEEILGYGDD
GLPR

Nucleotide

• Length: 255 bp

>AT25352 NC_015635:705464-705718 [Microlunatus phosphovorus NM-1]
ATGGCGTTGAACATCAAGGATCCGGAGACCGACCGGTTGGCGCGCGAACTGGCCGCTGCCACGGGTGAGTCGATCACGGT
GGCGGCGCGCCGGGCGATTGAGGAACGGCTCGAACGAGTTCGCGCTCGAGCCGACGCCGGTTCGGCAAGCGTCGATCTCG
GTGATCTGATCGAACGGGCGCGATCACGCCCTGATCTCGACGTACGTTCGGCTGAGGAGATCCTCGGGTACGGCGACGAC
GGTCTGCCTCGATGA

Structures

Toxin

• AlphaFold DB: F5XKW8

Antitoxin

• AlphaFold DB: F5XKW7