25351

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 2830163..2830796 Replicon chromosome
Accession NC_015634
Organism Bacillus coagulans 2-6

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag BCO26_RS14195 Protein ID WP_013860680.1
Coordinates 2830163..2830513 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag BCO26_RS14200 Protein ID WP_014096808.1
Coordinates 2830518..2830796 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCO26_RS14155 2825348..2826127 - 780 WP_013860672.1 RNA polymerase sigma factor SigB -
BCO26_RS14160 2826105..2826575 - 471 WP_013860673.1 anti-sigma B factor RsbW -
BCO26_RS14165 2826579..2826908 - 330 WP_013860674.1 anti-sigma factor antagonist -
BCO26_RS14170 2826945..2827988 - 1044 WP_013860675.1 PP2C family protein-serine/threonine phosphatase -
BCO26_RS14175 2828003..2828404 - 402 WP_013860676.1 anti-sigma regulatory factor -
BCO26_RS14180 2828409..2828765 - 357 WP_017553290.1 STAS domain-containing protein -
BCO26_RS14185 2828769..2829596 - 828 WP_041819511.1 RsbT co-antagonist protein RsbRA -
BCO26_RS14195 2830163..2830513 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BCO26_RS14200 2830518..2830796 - 279 WP_014096808.1 hypothetical protein Antitoxin
BCO26_RS14205 2830949..2832100 - 1152 WP_013860682.1 alanine racemase -
BCO26_RS14210 2832302..2833318 - 1017 WP_013860683.1 outer membrane lipoprotein carrier protein LolA -
BCO26_RS14215 2833459..2833809 - 351 WP_013860684.1 holo-ACP synthase -
BCO26_RS14220 2834091..2834690 + 600 WP_013860685.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T25351 WP_013860680.1 NC_015634:c2830513-2830163 [Bacillus coagulans 2-6]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T25351 NC_015634:c2830513-2830163 [Bacillus coagulans 2-6]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCTATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATCGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAGAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT25351 WP_014096808.1 NC_015634:c2830796-2830518 [Bacillus coagulans 2-6]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT25351 NC_015634:c2830796-2830518 [Bacillus coagulans 2-6]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAGTTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGCTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References