253493

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 263328..264007 Replicon chromosome
Accession NZ_CP102378
Organism Escherichia coli strain JB41

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag NR351_RS01255 Protein ID WP_000854672.1
Coordinates 263328..263669 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag NR351_RS01260 Protein ID WP_000070395.1
Coordinates 263690..264007 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR351_RS01230 (258677) 258677..259006 + 330 Protein_238 sigma factor-binding protein Crl -
NR351_RS01235 (259045) 259045..260100 - 1056 WP_000749863.1 phosphoporin PhoE -
NR351_RS01240 (260388) 260388..261491 + 1104 WP_001285288.1 glutamate 5-kinase -
NR351_RS01245 (261503) 261503..262756 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
NR351_RS01255 (263328) 263328..263669 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
NR351_RS01260 (263690) 263690..264007 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
NR351_RS01265 (264026) 264026..264247 - 222 WP_000691994.1 DUF987 domain-containing protein -
NR351_RS01270 (264256) 264256..264732 - 477 WP_000811693.1 RadC family protein -
NR351_RS01275 (264748) 264748..265206 - 459 WP_000211838.1 antirestriction protein -
NR351_RS01280 (265304) 265304..265543 - 240 WP_000194654.1 DUF905 family protein -
NR351_RS01285 (265620) 265620..266087 - 468 WP_001547765.1 protein YkfB -
NR351_RS01290 (266110) 266110..266553 - 444 WP_000824223.1 lipoprotein YafY -
NR351_RS01295 (266553) 266553..266729 - 177 WP_001285112.1 hypothetical protein -
NR351_RS01300 (266776) 266776..266967 - 192 Protein_251 DeoR family transcriptional regulator -
NR351_RS01305 (267184) 267184..268005 - 822 WP_000197389.1 DUF932 domain-containing protein -
NR351_RS01310 (268097) 268097..268960 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T253493 WP_000854672.1 NZ_CP102378:c263669-263328 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT253493 WP_000070395.1 NZ_CP102378:c264007-263690 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References