Detailed information of TA system TA025349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/YoeB-RelB
• Location: 1479706..1480240
• Replicon: chromosome
• Accession: NC_015602
• Organism: Lactobacillus kefiranofaciens ZW3

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: -
• Locus tag: WANG_RS07250
• Protein ID: WP_013854792.1
• Coordinates: 1479977..1480240 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: A0A656YAM3
• Locus tag: WANG_RS07245
• Protein ID: WP_013854791.1
• Coordinates: 1479706..1479993 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WANG_RS11865	1476362..1476595	-	234	WP_025084256.1	bacteriocin immunity protein	-
WANG_RS07225	1476570..1476767	-	198	WP_013854786.1	helix-turn-helix transcriptional regulator	-
WANG_RS07230	1476764..1477240	-	477	WP_013854787.1	hypothetical protein	-
WANG_RS07235	1477937..1479037	+	1101	WP_013854789.1	vitamin B12 independent methionine synthase	-
WANG_RS07240	1479100..1479576	+	477	WP_013854790.1	S-ribosylhomocysteine lyase	-
WANG_RS07245	1479706..1479993	+	288	WP_013854791.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
WANG_RS07250	1479977..1480240	+	264	WP_013854792.1	Txe/YoeB family addiction module toxin	Toxin
WANG_RS07255	1480525..1481850	-	1326	WP_013851263.1	IS200/IS605 family accessory protein TnpB-related protein	-
WANG_RS07260	1481880..1482488	-	609	WP_013851264.1	IS607 family transposase	-
WANG_RS12255	1483014..1483178	-	165	WP_013854793.1	hypothetical protein	-
WANG_RS12345	1483188..1483349	-	162	WP_013854794.1	hypothetical protein	-
WANG_RS07265	1483523..1485044	-	1522	Protein_1414	L-lactate permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1466407..1605355	138948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10486.95 Da
• Isoelectric Point: 9.3821

>T25349 WP_013854792.1 NC_015602:1479977-1480240 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
MLSWTDDGCDDYLNWQKQRQKKKIKRINKLITDIKRHPFEGMGKPEGLKNNLTGLWSRKIDAKNRIIYEYTNENVIIYSC
KDHYDDH

Nucleotide

• Length: 264 bp

>T25349 NC_015602:1479977-1480240 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
ATGCTAAGTTGGACTGATGATGGCTGTGACGACTACTTGAATTGGCAAAAGCAAAGGCAGAAAAAGAAAATAAAACGTAT
CAACAAATTAATTACAGACATTAAACGTCATCCCTTTGAAGGAATGGGCAAACCGGAAGGCTTAAAGAATAATCTAACAG
GCTTATGGTCTCGTAAAATTGATGCTAAAAACAGAATTATTTATGAGTATACAAATGAGAACGTTATAATCTATTCCTGC
AAAGATCATTATGATGACCATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10874.12 Da
• Isoelectric Point: 4.5296

>AT25349 WP_013854791.1 NC_015602:1479706-1479993 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
MDAVNYSNFRKDLKTYFKQVNDDSEPLIVTNKNPEDNVVVLSKDEYDALIETLAIQSNKYLMDKINRGRKQVAKAQTNQH
ELIDPDGEVSDAKLD

Nucleotide

• Length: 288 bp

>AT25349 NC_015602:1479706-1479993 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
ATGGATGCTGTAAACTATAGTAATTTTAGAAAAGATTTAAAAACCTACTTTAAGCAAGTAAACGATGATAGTGAGCCTTT
GATTGTAACTAATAAAAATCCAGAAGATAATGTCGTTGTCTTAAGCAAAGATGAATATGACGCTTTAATAGAAACCTTGG
CAATTCAATCTAATAAATATCTAATGGATAAAATTAATCGTGGACGCAAGCAAGTAGCTAAAGCACAAACTAATCAACAT
GAACTAATTGATCCAGATGGCGAGGTATCAGATGCTAAGTTGGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A656YAM3