Detailed information of TA system TA025347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 1159305..1160563
• Replicon: chromosome
• Accession: NC_015600
• Organism: Streptococcus pasteurianus ATCC 43144

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: F5X6Y6
• Locus tag: SGPB_RS05805
• Protein ID: WP_013851912.1
• Coordinates: 1159305..1160087 (-)
• Length: 261 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: SGPB_RS05810
• Protein ID: WP_013851913.1
• Coordinates: 1160087..1160563 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SGPB_RS05780	1154442..1156316	-	1875	WP_013851907.1	relaxase/mobilization nuclease domain-containing protein	-
SGPB_RS05785	1156303..1156668	-	366	WP_013851908.1	MobC family plasmid mobilization relaxosome protein	-
SGPB_RS05790	1156671..1157033	-	363	WP_013851909.1	hypothetical protein	-
SGPB_RS05795	1157377..1158519	-	1143	WP_013851910.1	AAA family ATPase	-
SGPB_RS05800	1158520..1159308	-	789	WP_013851911.1	hypothetical protein	-
SGPB_RS05805	1159305..1160087	-	783	WP_013851912.1	zeta toxin family protein	Toxin
SGPB_RS05810	1160087..1160563	-	477	WP_013851913.1	helix-turn-helix domain-containing protein	Antitoxin
SGPB_RS05815	1160633..1160923	-	291	WP_043910211.1	hypothetical protein	-
SGPB_RS05820	1160973..1161362	-	390	WP_013851914.1	hypothetical protein	-
SGPB_RS05825	1161359..1161586	-	228	WP_013851915.1	hypothetical protein	-
SGPB_RS05830	1161640..1162716	-	1077	WP_013851916.1	toprim domain-containing protein	-
SGPB_RS05835	1162756..1163394	-	639	WP_013851917.1	peptidylprolyl isomerase	-
SGPB_RS05840	1163490..1163780	-	291	Protein_1107	hypothetical protein	-
SGPB_RS10360	1163972..1164547	-	576	WP_013851918.1	TetR/AcrR family transcriptional regulator	-
SGPB_RS11175	1164645..1165499	+	855	WP_013851919.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1128292..1213737	85445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (30-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 261 a.a.
• Molecular weight: 29621.63 Da
• Isoelectric Point: 5.4776

>T25347 WP_013851912.1 NC_015600:c1160087-1159305 [Streptococcus pasteurianus ATCC 43144]
MRLEEFSEEEFQKALQRTIRALTRGKTILGQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLVLQE
KYGKDSVDYTKGFAGKMVEQLVDELSKRGYHLLIEGPLRTTQVPRQTAQLLASKGYQVSLAVIGTKPELSYLSTLVRYEE
LYAIDPNQARATPKEHHDGIVENLVDNLRELVREQLFDQIQIYQRDRTCIYDSETDEDSAAEVLQDCLFGEWSKVEEEML
KVGEEKLANLIDKNMIGSKK

Nucleotide

• Length: 783 bp

>T25347 NC_015600:c1160087-1159305 [Streptococcus pasteurianus ATCC 43144]
ATGAGATTGGAAGAATTTAGTGAGGAAGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCTAGGTCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGGGCAGGTAAGACAACGATACATCGTATCAAGCAAA
AAGAATTTCAAGGTAATATCATTATCATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGTACTGCAAGAA
AAGTATGGCAAGGACAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAA
ACGTGGTTATCATTTGTTAATTGAAGGGCCTTTACGAACCACTCAAGTTCCTCGTCAGACTGCTCAATTATTAGCTTCGA
AAGGATACCAAGTTTCCTTAGCTGTCATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGGTTCGTTACGAAGAA
CTTTATGCCATCGATCCCAATCAGGCCAGAGCAACACCCAAAGAACACCATGATGGGATTGTAGAGAACTTGGTTGATAA
CCTAAGGGAGTTAGTAAGAGAGCAACTCTTTGACCAGATTCAGATTTATCAAAGAGATAGAACTTGTATCTACGATTCTG
AAACTGATGAAGATTCCGCAGCAGAAGTTCTTCAAGACTGCCTCTTCGGAGAATGGAGTAAGGTCGAGGAGGAGATGTTG
AAGGTGGGAGAAGAGAAATTAGCTAACTTAATAGATAAGAATATGATTGGGAGTAAGAAATGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18315.76 Da
• Isoelectric Point: 4.4042

>AT25347 WP_013851913.1 NC_015600:c1160563-1160087 [Streptococcus pasteurianus ATCC 43144]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYQLTLKIE
VIKERGAAILSQLYRYQDSQDIAFDDETNPWILMSDDLAELINTKIYLVDTFDEIERYNGYLDGIERMLEMVHHRVVA

Nucleotide

• Length: 477 bp

>AT25347 NC_015600:c1160563-1160087 [Streptococcus pasteurianus ATCC 43144]
ATGATTGGAGACAATATTAAATCACTACGTCGAACACACGATTTAACACAACCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCTTAAGTCGTTATGAAAATGGGACTAGTACAGTTTCAACAGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTTGAAGATTACCAACTGACTTTAAAAATAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCACAGCTTTATAGATACCAGGATAGTCAAGATATTGCTTTTGATGATGA
AACAAATCCTTGGATTTTGATGAGTGATGATTTGGCTGAATTGATTAATACGAAAATATACCTTGTCGATACCTTCGATG
AAATAGAGCGTTATAATGGCTACTTAGACGGTATTGAGCGGATGTTAGAGATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: F5X6Y6