Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1299748..1300665 Replicon chromosome
Accession NZ_CP102350
Organism Bacillus velezensis strain PMC206

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag NRF13_RS07055 Protein ID WP_003154806.1
Coordinates 1299919..1300665 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag NRF13_RS07050 Protein ID WP_003154807.1
Coordinates 1299748..1299918 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRF13_RS07010 (NRF13_07010) 1294979..1296601 + 1623 WP_058905960.1 pyocin knob domain-containing protein -
NRF13_RS07015 (NRF13_07015) 1296614..1296985 + 372 WP_014304856.1 XkdW family protein -
NRF13_RS07020 (NRF13_07020) 1296991..1297188 + 198 WP_003154819.1 XkdX family protein -
NRF13_RS07025 (NRF13_07025) 1297245..1298006 + 762 WP_058905961.1 hypothetical protein -
NRF13_RS07030 (NRF13_07030) 1298058..1298321 + 264 WP_003154815.1 hemolysin XhlA family protein -
NRF13_RS07035 (NRF13_07035) 1298335..1298598 + 264 WP_003154813.1 phage holin -
NRF13_RS07040 (NRF13_07040) 1298612..1299490 + 879 WP_058905962.1 N-acetylmuramoyl-L-alanine amidase -
NRF13_RS07045 (NRF13_07045) 1299526..1299651 - 126 WP_003154809.1 hypothetical protein -
NRF13_RS07050 (NRF13_07050) 1299748..1299918 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
NRF13_RS07055 (NRF13_07055) 1299919..1300665 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
NRF13_RS07060 (NRF13_07060) 1300770..1301768 - 999 WP_003154805.1 inorganic phosphate transporter -
NRF13_RS07065 (NRF13_07065) 1301781..1302398 - 618 WP_003154804.1 DUF47 domain-containing protein -
NRF13_RS07070 (NRF13_07070) 1302684..1304000 - 1317 WP_003154801.1 amino acid permease -
NRF13_RS07075 (NRF13_07075) 1304323..1305273 + 951 WP_015388352.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T253466 WP_003154806.1 NZ_CP102350:c1300665-1299919 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT253466 WP_003154807.1 NZ_CP102350:c1299918-1299748 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References