253465

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 482364..483001 Replicon chromosome
Accession NZ_CP102350
Organism Bacillus velezensis strain PMC206

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NRF13_RS02580 Protein ID WP_003156187.1
Coordinates 482651..483001 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NRF13_RS02575 Protein ID WP_003156188.1
Coordinates 482364..482645 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRF13_RS02555 (NRF13_02555) 478730..479329 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NRF13_RS02560 (NRF13_02560) 479422..479787 + 366 WP_014304402.1 holo-ACP synthase -
NRF13_RS02565 (NRF13_02565) 479951..480958 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
NRF13_RS02570 (NRF13_02570) 481075..482244 + 1170 WP_058906584.1 alanine racemase -
NRF13_RS02575 (NRF13_02575) 482364..482645 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NRF13_RS02580 (NRF13_02580) 482651..483001 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NRF13_RS02585 (NRF13_02585) 483119..483940 + 822 WP_014304404.1 STAS domain-containing protein -
NRF13_RS02590 (NRF13_02590) 483945..484310 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NRF13_RS02595 (NRF13_02595) 484313..484714 + 402 WP_003156178.1 anti-sigma regulatory factor -
NRF13_RS02600 (NRF13_02600) 484726..485733 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
NRF13_RS02605 (NRF13_02605) 485797..486126 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NRF13_RS02610 (NRF13_02610) 486123..486605 + 483 WP_003156173.1 anti-sigma B factor RsbW -
NRF13_RS02615 (NRF13_02615) 486571..487359 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NRF13_RS02620 (NRF13_02620) 487359..487961 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T253465 WP_003156187.1 NZ_CP102350:482651-483001 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT253465 WP_003156188.1 NZ_CP102350:482364-482645 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References