Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 31679..32262 Replicon plasmid pWW1
Accession NC_015598
Organism Lactobacillus kefiranofaciens ZW3

Toxin (Protein)


Gene name mazF Uniprot ID A0A656Y774
Locus tag WANG_RS10445 Protein ID WP_025084310.1
Coordinates 31924..32262 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A656Y737
Locus tag WANG_RS10440 Protein ID WP_013851202.1
Coordinates 31679..31930 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WANG_RS10405 27387..27668 - 282 WP_013851193.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
WANG_RS10410 27675..27962 - 288 WP_013851194.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
WANG_RS10415 28195..29271 + 1077 WP_013851195.1 Fic family protein -
WANG_RS10420 29465..29710 + 246 WP_013851196.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
WANG_RS10425 29713..30042 + 330 WP_013851197.1 type II toxin-antitoxin system RelE/ParE family toxin -
WANG_RS10430 30200..30790 + 591 WP_013851198.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -
WANG_RS10435 30821..31021 + 201 Protein_26 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
WANG_RS10440 31679..31930 + 252 WP_013851202.1 hypothetical protein Antitoxin
WANG_RS10445 31924..32262 + 339 WP_025084310.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
WANG_RS10450 32761..33795 + 1035 WP_013851204.1 IS30 family transposase -
WANG_RS10460 33951..35129 + 1179 Protein_30 IS256 family transposase -
WANG_RS10465 35324..36601 - 1278 WP_013851207.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..194769 194769


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-107)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13056.26 Da        Isoelectric Point: 9.8423

>T25345 WP_025084310.1 NC_015598:31924-32262 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
MVIKQGSVLLMDLSPKQGHEQSGKRPYLYLSYQGVEKYAHIAIFAPISTTKRRYPLYRNLPDSCKTKGVVMLDQLVTVDY
HNRKHQFLEQLPDDYMEKLLKIVKVVFQKDPS

Download         Length: 339 bp

>T25345 NC_015598:31924-32262 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
ATGGTAATTAAACAAGGATCCGTTTTATTGATGGATTTAAGTCCTAAGCAGGGACATGAACAATCCGGCAAGCGTCCATA
TTTATATTTAAGTTATCAAGGTGTTGAAAAATATGCCCATATAGCGATTTTTGCTCCAATTTCAACTACGAAACGGCGAT
ATCCACTATATAGAAATCTACCAGATTCTTGTAAAACTAAAGGCGTAGTTATGTTAGATCAACTTGTAACTGTGGATTAT
CATAATCGCAAACATCAATTTTTAGAGCAATTACCTGATGACTATATGGAAAAGTTGCTAAAGATTGTAAAGGTAGTATT
TCAAAAAGATCCTTCTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9503.84 Da        Isoelectric Point: 9.6573

>AT25345 WP_013851202.1 NC_015598:31679-31930 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
MITATISKWGNSKGIRLNQAVLQALNAKTGDHLNFKIEDNRVIITKKEPKHELTFEELFKDYKGGSFDTKISLPDERVGN
EKW

Download         Length: 252 bp

>AT25345 NC_015598:31679-31930 [Lactobacillus kefiranofaciens subsp. kefiranofaciens]
ATGATAACTGCTACAATTTCAAAATGGGGTAATTCTAAAGGTATTAGACTTAATCAGGCCGTTCTACAAGCATTAAATGC
AAAAACTGGTGATCATTTAAATTTTAAAATTGAGGATAATAGGGTAATTATCACTAAGAAGGAACCAAAACATGAACTTA
CTTTTGAAGAACTTTTTAAGGATTATAAAGGTGGTTCATTTGATACAAAGATTTCATTACCAGATGAAAGAGTGGGCAAT
GAAAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656Y774

Antitoxin

Source ID Structure
AlphaFold DB A0A656Y737

References