Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4541229..4541770 Replicon chromosome
Accession NZ_CP102344
Organism Mycolicibacterium smegmatis strain LM12

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag NQ425_RS21615 Protein ID WP_003895797.1
Coordinates 4541441..4541770 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag NQ425_RS21610 Protein ID WP_011729843.1
Coordinates 4541229..4541438 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQ425_RS21585 (NQ425_21585) 4536500..4537270 - 771 WP_011729837.1 SDR family oxidoreductase -
NQ425_RS21590 (NQ425_21590) 4537807..4538208 - 402 Protein_4290 cupin domain-containing protein -
NQ425_RS21595 (NQ425_21595) 4538238..4539158 - 921 WP_011729840.1 NADP-dependent oxidoreductase -
NQ425_RS21600 (NQ425_21600) 4539155..4539439 - 285 WP_011729841.1 hypothetical protein -
NQ425_RS21605 (NQ425_21605) 4539701..4541068 + 1368 WP_014878037.1 dihydrolipoyl dehydrogenase -
NQ425_RS21610 (NQ425_21610) 4541229..4541438 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
NQ425_RS21615 (NQ425_21615) 4541441..4541770 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NQ425_RS21620 (NQ425_21620) 4541813..4542376 - 564 WP_014878038.1 helix-turn-helix domain-containing protein -
NQ425_RS21625 (NQ425_21625) 4542482..4543417 + 936 WP_003895799.1 alpha/beta fold hydrolase -
NQ425_RS21630 (NQ425_21630) 4543414..4545126 + 1713 WP_011729844.1 NAD(P)/FAD-dependent oxidoreductase -
NQ425_RS21640 (NQ425_21640) 4545343..4545723 + 381 WP_029104585.1 phage holin family protein -
NQ425_RS21645 (NQ425_21645) 4545741..4546766 + 1026 WP_014878040.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T253448 WP_003895797.1 NZ_CP102344:4541441-4541770 [Mycolicibacterium smegmatis]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT253448 WP_011729843.1 NZ_CP102344:4541229-4541438 [Mycolicibacterium smegmatis]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References