Detailed information of TA system TA025344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 225090..225670
• Replicon: plasmid pSINME01
• Accession: NC_015597
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SINME_RS33755
• Protein ID: WP_041915340.1
• Coordinates: 225287..225670 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XCL0
• Locus tag: SINME_RS33750
• Protein ID: WP_013851145.1
• Coordinates: 225090..225290 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS33705	220215..220571	-	357	WP_013851142.1	hypothetical protein	-
SINME_RS33710	220683..220856	-	174	Protein_228	adenylate/guanylate cyclase domain-containing protein	-
SINME_RS33715	221197..221741	+	545	Protein_229	dienelactone hydrolase family protein	-
SINME_RS33725	222059..222205	-	147	WP_017266778.1	hypothetical protein	-
SINME_RS33730	222299..223027	-	729	WP_013851143.1	class I SAM-dependent methyltransferase	-
SINME_RS37655	223066..223389	-	324	WP_017266777.1	DUF1127 domain-containing protein	-
SINME_RS33740	223485..224321	+	837	WP_013851144.1	helix-turn-helix transcriptional regulator	-
SINME_RS37660	224398..224799	-	402	WP_014531161.1	GFA family protein	-
SINME_RS33750	225090..225290	+	201	WP_013851145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SINME_RS33755	225287..225670	+	384	WP_041915340.1	type II toxin-antitoxin system VapC family toxin	Toxin
SINME_RS33765	226476..227888	+	1413	WP_013851149.1	PhoPQ-activated pathogenicity-related family protein	-
SINME_RS33770	228480..228911	+	432	WP_013851150.1	ParB N-terminal domain-containing protein	-
SINME_RS33775	228908..230281	+	1374	WP_013851151.1	Y4yA family PLP-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256269	256269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14246.55 Da
• Isoelectric Point: 7.1866

>T25344 WP_041915340.1 NC_015597:225287-225670 [Sinorhizobium meliloti AK83]
VILADISIWIDHFRHTDAELRRIIEGDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Nucleotide

• Length: 384 bp

>T25344 NC_015597:225287-225670 [Sinorhizobium meliloti AK83]
GTGATTCTTGCCGACATTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGGCGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7096.22 Da
• Isoelectric Point: 11.1107

>AT25344 WP_013851145.1 NC_015597:225090-225290 [Sinorhizobium meliloti AK83]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT25344 NC_015597:225090-225290 [Sinorhizobium meliloti AK83]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W6MID2