Detailed information of TA system TA025343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 875..1506
• Replicon: plasmid pSINME01
• Accession: NC_015597
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SINME_RS32645
• Protein ID: WP_013851004.1
• Coordinates: 875..1261 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SINME_RS32650
• Protein ID: WP_041915309.1
• Coordinates: 1258..1506 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS40165	295..462	+	168	WP_013851003.1	hypothetical protein	-
SINME_RS32645	875..1261	-	387	WP_013851004.1	type II toxin-antitoxin system VapC family toxin	Toxin
SINME_RS32650	1258..1506	-	249	WP_041915309.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SINME_RS32655	1628..2790	-	1163	Protein_3	tyrosine-type recombinase/integrase	-
SINME_RS32660	2888..4010	+	1123	Protein_4	DUF1612 and helix-turn-helix domain-containing protein	-
SINME_RS32665	4212..5187	+	976	Protein_5	IS5 family transposase	-
SINME_RS37385	5290..5587	+	298	Protein_6	hypothetical protein	-
SINME_RS37390	5594..5722	+	129	Protein_7	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256269	256269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14037.99 Da
• Isoelectric Point: 5.1512

>T25343 WP_013851004.1 NC_015597:c1261-875 [Sinorhizobium meliloti AK83]
VIVDTSALVAILYREPQAARFVNYIHDAETTRISVASYIELSMVVESQLGPDGMRQAEAFFRRAGIIVEPVTLEHGELAR
QAFLDFGKGRHKAGLNFGDCFAYALAKASGEPLLFKGNDFSQTDVLTV

Nucleotide

• Length: 387 bp

>T25343 NC_015597:c1261-875 [Sinorhizobium meliloti AK83]
GTGATTGTCGACACCTCCGCGCTGGTCGCCATCCTTTACCGCGAGCCCCAAGCGGCACGCTTTGTCAACTATATCCACGA
CGCCGAAACCACTCGGATCAGCGTCGCTAGTTATATCGAGTTGTCCATGGTGGTGGAAAGTCAGCTCGGACCTGACGGTA
TGCGCCAGGCCGAAGCCTTCTTCCGGCGGGCCGGCATCATTGTTGAGCCGGTCACGCTTGAACATGGCGAACTGGCGCGT
CAGGCTTTTCTCGACTTCGGCAAGGGCCGGCACAAGGCCGGTCTCAATTTCGGTGATTGCTTCGCCTACGCCCTCGCCAA
AGCGTCTGGCGAACCGTTACTGTTCAAGGGCAATGACTTTTCTCAAACTGATGTCCTGACCGTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9158.33 Da
• Isoelectric Point: 7.4693

>AT25343 WP_041915309.1 NC_015597:c1506-1258 [Sinorhizobium meliloti AK83]
VSLSIKDPEAHRLAQAISHATGESMTRVVTNALRERLAKIERRKGRASVEELLAIADRAATHVKHPYDDHADFLYDENGI
PK

Nucleotide

• Length: 249 bp

>AT25343 NC_015597:c1506-1258 [Sinorhizobium meliloti AK83]
GTGAGCCTCAGCATCAAGGATCCAGAAGCCCATCGTCTGGCGCAGGCGATTTCCCACGCGACGGGGGAGAGCATGACCCG
CGTCGTCACCAATGCACTGCGCGAGCGCCTTGCCAAGATTGAACGCCGAAAGGGCCGCGCCAGCGTTGAGGAACTGCTGG
CGATCGCCGACCGTGCCGCCACGCACGTCAAACACCCTTATGACGATCATGCCGACTTTCTTTACGACGAGAACGGCATT
CCCAAGTGA