Detailed information of TA system TA025341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 775240..775604
• Replicon: chromosome
• Accession: NC_015596
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SINME_RS40245
• Protein ID: WP_028005487.1
• Coordinates: 775240..775443 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SINME_RS35595
• Protein ID: WP_201766980.1
• Coordinates: 775440..775604 (-)
• Length: 55 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS22050	770753..771034	+	282	WP_010976128.1	DUF1236 domain-containing protein	-
SINME_RS22055	771164..771427	+	264	WP_013850410.1	hypothetical protein	-
SINME_RS22060	771724..772200	+	477	WP_013850411.1	CBS domain-containing protein	-
SINME_RS22065	772194..772454	+	261	WP_010976125.1	DUF982 domain-containing protein	-
SINME_RS22070	772498..772800	-	303	WP_013850412.1	hypothetical protein	-
SINME_RS22075	773580..774940	+	1361	Protein_723	group II intron reverse transcriptase/maturase	-
SINME_RS40240	774893..775240	-	348	WP_074935087.1	type II toxin-antitoxin system PemK/MazF family toxin	-
SINME_RS40245	775240..775443	-	204	WP_028005487.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SINME_RS35595	775440..775604	-	165	WP_201766980.1	DUF3018 family protein	Antitoxin
SINME_RS22085	775758..776540	-	783	WP_013850413.1	SDR family oxidoreductase	-
SINME_RS22090	776537..776908	-	372	WP_013850414.1	hypothetical protein	-
SINME_RS22095	777012..777440	-	429	WP_013850415.1	low affinity iron permease family protein	-
SINME_RS22100	777602..777850	-	249	WP_013850416.1	DUF982 domain-containing protein	-
SINME_RS22105	777828..778109	-	282	WP_003526277.1	DUF982 domain-containing protein	-
SINME_RS39860	778219..778470	-	252	WP_013850417.1	DUF982 domain-containing protein	-
SINME_RS22115	778825..779922	+	1098	WP_013850418.1	DUF475 domain-containing protein	-
SINME_RS22120	780129..780473	+	345	WP_017266930.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-62)

Antitoxin

• Antitoxin MazE-like (1-52)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7300.49 Da
• Isoelectric Point: 5.7105

>T25341 WP_028005487.1 NC_015596:c775443-775240 [Sinorhizobium meliloti AK83]
MKRGDLVTIALPVDFGKPRPAPIIQADLFEDTGTVTVLLVSEALLDAPLLWPTVRPTPENGLGNRHR

Nucleotide

• Length: 204 bp

>T25341 NC_015596:c775443-775240 [Sinorhizobium meliloti AK83]
ATGAAGCGTGGAGATCTCGTTACCATCGCCCTGCCGGTGGACTTCGGGAAGCCCCGGCCTGCCCCGATCATCCAGGCTGA
TTTGTTCGAGGACACCGGCACGGTGACTGTGCTGCTGGTGTCGGAAGCGTTGCTCGATGCCCCTCTGTTGTGGCCAACGG
TACGACCGACGCCGGAGAATGGGCTTGGTAACCGTCACAGATAA

Antitoxin

Protein

• Length: 55 a.a.
• Molecular weight: 5753.43 Da
• Isoelectric Point: 4.5003

>AT25341 WP_201766980.1 NC_015596:c775604-775440 [Sinorhizobium meliloti AK83]
MRAAGLRPVQIWIPDNRRPGFADECRRQAAAVAAADAAARDLDAFIDAALSDDA

Nucleotide

• Length: 165 bp

>AT25341 NC_015596:c775604-775440 [Sinorhizobium meliloti AK83]
CTGCGGGCGGCCGGGTTGCGCCCCGTGCAAATATGGATTCCGGATAACCGCCGCCCAGGTTTTGCCGACGAGTGCCGGCG
CCAGGCGGCGGCGGTCGCCGCGGCTGATGCTGCCGCCCGCGACCTGGATGCCTTCATTGACGCCGCTCTGTCCGATGATG
CATGA