Detailed information of TA system TA025340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 423645..424179
• Replicon: chromosome
• Accession: NC_015596
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: SINME_RS20480
• Protein ID: WP_013850205.1
• Coordinates: 423645..423941 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: SINME_RS20485
• Protein ID: WP_003527850.1
• Coordinates: 423931..424179 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS20455	419087..420490	-	1404	WP_041915145.1	sodium:alanine symporter family protein	-
SINME_RS20460	421024..421233	-	210	WP_003527838.1	dodecin family protein	-
SINME_RS20465	421523..421969	+	447	WP_013850202.1	DNA-binding protein	-
SINME_RS20470	421973..422158	-	186	WP_013850203.1	hypothetical protein	-
SINME_RS20475	422325..422723	-	399	WP_010974988.1	hypothetical protein	-
SINME_RS35495	423304..423564	+	261	Protein_402	helix-turn-helix domain containing protein	-
SINME_RS20480	423645..423941	-	297	WP_013850205.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SINME_RS20485	423931..424179	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
SINME_RS38965	424260..424427	-	168	WP_017266563.1	hypothetical protein	-
SINME_RS20495	424783..424908	+	126	Protein_406	IS481 family transposase	-
SINME_RS20500	424992..425786	-	795	WP_013850206.1	methyltransferase domain-containing protein	-
SINME_RS20505	425790..426590	-	801	WP_010974981.1	ABC transporter ATP-binding protein	-
SINME_RS20510	426587..427630	-	1044	WP_010974980.1	iron ABC transporter permease	-
SINME_RS20515	427627..428712	-	1086	WP_013850207.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	424717..424908	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11471.14 Da
• Isoelectric Point: 10.7401

>T25340 WP_013850205.1 NC_015596:c423941-423645 [Sinorhizobium meliloti AK83]
MATERPFRLAPAARADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T25340 NC_015596:c423941-423645 [Sinorhizobium meliloti AK83]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAGGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT25340 WP_003527850.1 NC_015596:c424179-423931 [Sinorhizobium meliloti AK83]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT25340 NC_015596:c424179-423931 [Sinorhizobium meliloti AK83]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0