Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4263443..4264019 Replicon chromosome
Accession NZ_CP102247
Organism Enterobacter asburiae strain R_A5.MM

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag NQ230_RS20265 Protein ID WP_010427219.1
Coordinates 4263732..4264019 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag NQ230_RS20260 Protein ID WP_257258848.1
Coordinates 4263443..4263745 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQ230_RS20240 (NQ230_20240) 4259748..4260293 + 546 WP_063143257.1 YfaZ family outer membrane protein -
NQ230_RS20245 (NQ230_20245) 4260461..4261831 - 1371 WP_257258844.1 NAD-dependent succinate-semialdehyde dehydrogenase -
NQ230_RS20250 (NQ230_20250) 4261983..4262726 + 744 WP_257258845.1 AraC family transcriptional regulator -
NQ230_RS20255 (NQ230_20255) 4262774..4263412 + 639 WP_257258847.1 LysE family translocator -
NQ230_RS20260 (NQ230_20260) 4263443..4263745 - 303 WP_257258848.1 BrnA antitoxin family protein Antitoxin
NQ230_RS20265 (NQ230_20265) 4263732..4264019 - 288 WP_010427219.1 BrnT family toxin Toxin
NQ230_RS20270 (NQ230_20270) 4264190..4265461 + 1272 WP_257258849.1 DUF445 domain-containing protein -
NQ230_RS20275 (NQ230_20275) 4265458..4266534 - 1077 WP_257258850.1 DUF2955 domain-containing protein -
NQ230_RS20280 (NQ230_20280) 4266524..4267591 - 1068 WP_121424942.1 HlyD family secretion protein -
NQ230_RS20285 (NQ230_20285) 4267588..4268058 - 471 WP_257258851.1 MarR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4268283..4269425 1142


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T253396 WP_010427219.1 NZ_CP102247:c4264019-4263732 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11377.01 Da        Isoelectric Point: 10.2483

>AT253396 WP_257258848.1 NZ_CP102247:c4263745-4263443 [Enterobacter asburiae]
MSMVKHKRGSAPEVSARREAELKALAEKSEAEIDYSDIPATADEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G892

Antitoxin

Source ID Structure

References