Detailed information of TA system TA025339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 72903..73570
• Replicon: chromosome
• Accession: NC_015596
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SINME_RS18820
• Protein ID: WP_013849979.1
• Coordinates: 73127..73570 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92WE1
• Locus tag: SINME_RS18815
• Protein ID: WP_010975287.1
• Coordinates: 72903..73130 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS18795	68297..69178	-	882	WP_013849975.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
SINME_RS18800	69296..70588	-	1293	WP_013849976.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
SINME_RS18805	70957..72246	+	1290	WP_013849977.1	SDR family oxidoreductase	-
SINME_RS18810	72246..72719	+	474	WP_013849978.1	DUF2269 family protein	-
SINME_RS18815	72903..73130	+	228	WP_010975287.1	hypothetical protein	Antitoxin
SINME_RS18820	73127..73570	+	444	WP_013849979.1	PIN domain-containing protein	Toxin
SINME_RS18825	73586..74524	-	939	WP_013849980.1	choline ABC transporter substrate-binding protein	-
SINME_RS18830	74593..75423	-	831	WP_013849981.1	SDR family oxidoreductase	-
SINME_RS18835	75420..75917	-	498	WP_013849982.1	acetone carboxylase subunit gamma	-
SINME_RS18840	75921..78113	-	2193	WP_013849983.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15650.94 Da
• Isoelectric Point: 6.6378

>T25339 WP_013849979.1 NC_015596:73127-73570 [Sinorhizobium meliloti AK83]
VTFLLDVNVLIALIDPSHIGHDDAHEWFASIGQTTWATCPITENGVIRIVGNPEYPNSPGSPLPVMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Nucleotide

• Length: 444 bp

>T25339 NC_015596:73127-73570 [Sinorhizobium meliloti AK83]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCACGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATTCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8174.39 Da
• Isoelectric Point: 9.1096

>AT25339 WP_010975287.1 NC_015596:72903-73130 [Sinorhizobium meliloti AK83]
MRTTLAIDDDVLIAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Nucleotide

• Length: 228 bp

>AT25339 NC_015596:72903-73130 [Sinorhizobium meliloti AK83]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCGCCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q92WE1