Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1939914..1940504 Replicon chromosome
Accession NZ_CP102246
Organism Enterobacter cloacae complex sp. R_G8

Toxin (Protein)


Gene name pemK Uniprot ID A0A427KSR1
Locus tag NQ842_RS09165 Protein ID WP_047360978.1
Coordinates 1940172..1940504 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A427KRT2
Locus tag NQ842_RS09160 Protein ID WP_020687575.1
Coordinates 1939914..1940171 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQ842_RS09135 (NQ842_09135) 1935248..1936198 + 951 WP_046888385.1 HTH-type transcriptional regulator Cbl -
NQ842_RS09140 (NQ842_09140) 1936241..1936849 - 609 WP_257256759.1 glutathione S-transferase family protein -
NQ842_RS09145 (NQ842_09145) 1937020..1937913 + 894 WP_257256760.1 transcriptional regulator GcvA -
NQ842_RS09150 (NQ842_09150) 1938040..1938969 - 930 WP_196372580.1 LysR family transcriptional regulator -
NQ842_RS09155 (NQ842_09155) 1939111..1939500 + 390 WP_257256761.1 RidA family protein -
NQ842_RS09160 (NQ842_09160) 1939914..1940171 + 258 WP_020687575.1 antitoxin Antitoxin
NQ842_RS09165 (NQ842_09165) 1940172..1940504 + 333 WP_047360978.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NQ842_RS09170 (NQ842_09170) 1940743..1944777 - 4035 WP_257256763.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1929623..1944777 15154


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-85)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11797.68 Da        Isoelectric Point: 10.5834

>T253381 WP_047360978.1 NZ_CP102246:1940172-1940504 [Enterobacter cloacae complex sp. R_G8]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKVTRLPVVVPVTSGGNFARTAGFTVSLDGAGTKTMGIIRCDQPRTI
DMAARNGKRLERVPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9437.90 Da        Isoelectric Point: 4.3556

>AT253381 WP_020687575.1 NZ_CP102246:1939914-1940171 [Enterobacter cloacae complex sp. R_G8]
MYTTRLKKVGGSIMLAVPPAVLKTLELSIDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAT
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A427KSR1

Antitoxin

Source ID Structure
AlphaFold DB A0A427KRT2

References