Detailed information of TA system TA025338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 115631..116162
• Replicon: plasmid pSPHCH01
• Accession: NC_015595
• Organism: Sphingobium chlorophenolicum L-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: SPHCH_RS20840
• Protein ID: WP_004212690.1
• Coordinates: 115631..115912 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: SPHCH_RS20845
• Protein ID: WP_004212689.1
• Coordinates: 115899..116162 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPHCH_RS20815	111683..112156	+	474	WP_007683361.1	DUF305 domain-containing protein	-
SPHCH_RS20820	112215..113750	+	1536	WP_007683359.1	PAS domain S-box protein	-
SPHCH_RS20825	113740..114363	+	624	WP_007683357.1	response regulator transcription factor	-
SPHCH_RS20830	114445..114852	+	408	WP_007683354.1	response regulator	-
SPHCH_RS20835	114915..115265	-	351	WP_007683352.1	magnesium transporter	-
SPHCH_RS20840	115631..115912	-	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SPHCH_RS20845	115899..116162	-	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SPHCH_RS20850	116547..116984	-	438	WP_007683347.1	metal-binding protein	-
SPHCH_RS20855	117064..118305	-	1242	WP_007683345.1	MFS transporter	-
SPHCH_RS20860	118299..119285	-	987	WP_009823975.1	copper resistance protein B	-
SPHCH_RS20865	119282..120988	-	1707	WP_007683343.1	copper resistance system multicopper oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..123733	123733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T25338 WP_004212690.1 NC_015595:c115912-115631 [Sphingobium chlorophenolicum L-1]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T25338 NC_015595:c115912-115631 [Sphingobium chlorophenolicum L-1]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT25338 WP_004212689.1 NC_015595:c116162-115899 [Sphingobium chlorophenolicum L-1]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT25338 NC_015595:c116162-115899 [Sphingobium chlorophenolicum L-1]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968