Detailed information of TA system TA025336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 56789..57342
• Replicon: plasmid pSINME02
• Accession: NC_015592
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SINME_RS34175
• Protein ID: WP_013845961.1
• Coordinates: 56789..57082 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SINME_RS34180
• Protein ID: WP_013845962.1
• Coordinates: 57070..57342 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS34165	54109..55053	+	945	WP_013845958.1	spore coat U domain-containing protein	-
SINME_RS37730	55180..55362	-	183	WP_013845959.1	hypothetical protein	-
SINME_RS34170	56026..56562	+	537	WP_013845960.1	hypothetical protein	-
SINME_RS34175	56789..57082	-	294	WP_013845961.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SINME_RS34180	57070..57342	-	273	WP_013845962.1	CopG family ribbon-helix-helix protein	Antitoxin
SINME_RS37735	57511..57804	-	294	WP_013845963.1	hypothetical protein	-
SINME_RS37740	58025..58396	+	372	WP_107591870.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SINME_RS34195	58410..58706	-	297	WP_050812828.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SINME_RS34200	58711..59184	-	474	WP_013845966.1	hypothetical protein	-
SINME_RS34205	59398..60108	-	711	WP_013845967.1	replication protein A	-
SINME_RS39635	60304..60435	-	132	WP_107591872.1	ribbon-helix-helix protein, CopG family	-
SINME_RS34210	60979..62019	+	1041	WP_041915382.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..70499	70499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10781.26 Da
• Isoelectric Point: 5.1693

>T25336 WP_013845961.1 NC_015592:c57082-56789 [Sinorhizobium meliloti AK83]
VPQVIVTEGAAEGLERCRRFLATKAPEAARRAGQAIERQFLLLETAPDIGRPFPEAPELRELVIAFGDSGYVALYRHEPA
DDAVYILAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T25336 NC_015592:c57082-56789 [Sinorhizobium meliloti AK83]
GTGCCACAAGTAATCGTCACCGAAGGCGCTGCGGAAGGCTTGGAGCGATGCCGGCGCTTCCTGGCCACTAAGGCCCCGGA
AGCCGCCAGACGGGCCGGCCAGGCCATCGAGCGGCAATTCCTGCTTCTGGAGACGGCGCCCGACATTGGCCGGCCGTTCC
CCGAAGCGCCTGAGCTGCGCGAGCTGGTGATTGCCTTCGGGGACTCCGGCTATGTGGCCCTGTACCGTCACGAGCCGGCC
GACGATGCGGTGTATATTCTGGCCTTCCGGCACCAGAAAGAAGCGGGCTACTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10561.83 Da
• Isoelectric Point: 6.7205

>AT25336 WP_013845962.1 NC_015592:c57342-57070 [Sinorhizobium meliloti AK83]
MATSLKIDDTLKSRVQQLASQRRRSSHWIMLEAIQQYVEREEARESFKQEALASWAAYKETGRHLTGEEVRTWLTTWGTD
DEKPVPECHK

Nucleotide

• Length: 273 bp

>AT25336 NC_015592:c57342-57070 [Sinorhizobium meliloti AK83]
ATGGCAACGTCTCTCAAGATCGACGACACACTGAAAAGTCGCGTCCAGCAGCTCGCCAGCCAGCGCCGCCGCTCGTCTCA
CTGGATCATGCTCGAAGCGATCCAACAGTACGTTGAGCGCGAGGAAGCACGCGAGAGCTTCAAGCAGGAGGCCTTGGCGT
CCTGGGCCGCGTACAAGGAAACCGGTCGCCACCTGACCGGCGAAGAAGTCCGCACCTGGTTGACTACCTGGGGTACCGAT
GACGAAAAGCCGGTGCCAGAGTGCCACAAGTAA