Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 95686..96211 Replicon plasmid pS270V-2
Accession NZ_CP102196
Organism Klebsiella pneumoniae strain S270v

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag NQZ65_RS28910 Protein ID WP_001159871.1
Coordinates 95686..95991 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag NQZ65_RS28915 Protein ID WP_000813634.1
Coordinates 95993..96211 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQZ65_RS28870 (91438) 91438..91665 + 228 WP_001254386.1 conjugal transfer relaxosome protein TraY -
NQZ65_RS28875 (91699) 91699..92064 + 366 WP_021519752.1 type IV conjugative transfer system pilin TraA -
NQZ65_RS28880 (92079) 92079..92390 + 312 WP_000012106.1 type IV conjugative transfer system protein TraL -
NQZ65_RS28885 (92412) 92412..92978 + 567 WP_000399794.1 type IV conjugative transfer system protein TraE -
NQZ65_RS28890 (92965) 92965..93693 + 729 WP_001230787.1 type-F conjugative transfer system secretin TraK -
NQZ65_RS28895 (93693) 93693..94430 + 738 Protein_133 conjugal transfer protein TraB -
NQZ65_RS28900 (94482) 94482..95186 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
NQZ65_RS28905 (95251) 95251..95685 - 435 Protein_135 site-specific integrase -
NQZ65_RS28910 (95686) 95686..95991 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
NQZ65_RS28915 (95993) 95993..96211 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NQZ65_RS28920 (96770) 96770..97696 + 927 WP_001290414.1 hypothetical protein -
NQZ65_RS28925 (97746) 97746..98000 + 255 WP_000678528.1 hypothetical protein -
NQZ65_RS28930 (98498) 98498..98842 + 345 WP_000792769.1 hypothetical protein -
NQZ65_RS28935 (99016) 99016..100017 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
NQZ65_RS28940 (100590) 100590..100880 - 291 WP_000648897.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / blaKPC-2 / blaSHV-12 / cmlA1 / qacE / blaCTX-M-14 - 1..154868 154868
- flank IS/Tn - - 94482..95186 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T253348 WP_001159871.1 NZ_CP102196:c95991-95686 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT253348 WP_000813634.1 NZ_CP102196:c96211-95993 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References