Detailed information of TA system TA025333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 323298..323896
• Replicon: chromosome
• Accession: NC_015591
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SINME_RS27755
• Protein ID: WP_013845238.1
• Coordinates: 323603..323896 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: SINME_RS27750
• Protein ID: WP_010967242.1
• Coordinates: 323298..323606 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS27735	319205..320938	-	1734	WP_013845235.1	hypothetical protein	-
SINME_RS36330	321399..321602	+	204	WP_013845236.1	cold-shock protein	-
SINME_RS39235	321614..321904	-	291	Protein_302	HU family DNA-binding protein	-
SINME_RS27745	322075..323169	+	1095	WP_013845237.1	IS1595 family transposase	-
SINME_RS27750	323298..323606	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SINME_RS27755	323603..323896	+	294	WP_013845238.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
SINME_RS27760	324158..325357	-	1200	WP_013845239.1	NAD-dependent formate dehydrogenase	-
SINME_RS36335	325604..325861	+	258	WP_074935271.1	hypothetical protein	-
SINME_RS27765	325997..326499	+	503	Protein_308	cupin domain-containing protein	-
SINME_RS27770	326861..327610	-	750	WP_033049384.1	carbonic anhydrase	-
SINME_RS27775	327782..328373	-	592	Protein_310	polysaccharide lyase family 1 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	322075..323169	1094

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11202.84 Da
• Isoelectric Point: 6.4735

>T25333 WP_013845238.1 NC_015591:323603-323896 [Sinorhizobium meliloti AK83]
MRLVWARYALDDRDTIFSSIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T25333 NC_015591:323603-323896 [Sinorhizobium meliloti AK83]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTCCATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT25333 WP_010967242.1 NC_015591:323298-323606 [Sinorhizobium meliloti AK83]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT25333 NC_015591:323298-323606 [Sinorhizobium meliloti AK83]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTTCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Antitoxin

• AlphaFold DB: Q930F1