Detailed information of TA system TA025332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 297911..298446
• Replicon: chromosome
• Accession: NC_015591
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SINME_RS36320
• Protein ID: WP_014989489.1
• Coordinates: 297911..298186 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N8JT21
• Locus tag: SINME_RS27640
• Protein ID: WP_013845222.1
• Coordinates: 298186..298446 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS27610	293299..294246	+	948	WP_013845217.1	LysR family transcriptional regulator	-
SINME_RS27615	294402..294707	-	306	Protein_277	hypothetical protein	-
SINME_RS27625	295420..295857	+	438	WP_013845218.1	VOC family protein	-
SINME_RS27630	296360..296659	+	300	WP_013845219.1	hypothetical protein	-
SINME_RS27635	296708..297883	-	1176	WP_013845220.1	site-specific integrase	-
SINME_RS36320	297911..298186	-	276	WP_014989489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SINME_RS27640	298186..298446	-	261	WP_013845222.1	ribbon-helix-helix domain-containing protein	Antitoxin
SINME_RS27645	298601..298810	-	210	Protein_283	transposase	-
SINME_RS27650	298818..299237	-	420	WP_013845223.1	type II toxin-antitoxin system VapC family toxin	-
SINME_RS27655	299234..299476	-	243	WP_013845224.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
SINME_RS27660	299907..301574	+	1668	WP_013845225.1	transposase	-
SINME_RS27665	301577..301897	-	321	WP_013845226.1	hypothetical protein	-
SINME_RS27670	301954..302523	-	570	WP_013845227.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	298601..298828	227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10895.46 Da
• Isoelectric Point: 9.9707

>T25332 WP_014989489.1 NC_015591:c298186-297911 [Sinorhizobium meliloti AK83]
MKLEWTSKAVADLGRLYDFLAPVNRQAAARTVQSWTSAPARLLEQPRIGERLEEFDPREVRRILVGHYEIRYEIRQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T25332 NC_015591:c298186-297911 [Sinorhizobium meliloti AK83]
ATGAAGCTTGAGTGGACGAGTAAGGCAGTCGCCGATCTTGGGCGCCTCTATGATTTCCTGGCACCGGTAAATCGGCAAGC
TGCGGCTCGTACTGTGCAATCATGGACGAGCGCTCCAGCACGCCTGCTCGAACAACCTCGCATCGGCGAACGGCTGGAGG
AATTCGACCCTCGAGAGGTTCGGCGAATTCTCGTTGGCCATTACGAAATACGATATGAGATCCGGCAGTCGACGATCTAC
GTGCTGCGGCTTTGGCATACGCGTGAAGACCGTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9652.01 Da
• Isoelectric Point: 4.8182

>AT25332 WP_013845222.1 NC_015591:c298446-298186 [Sinorhizobium meliloti AK83]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDAGRVIDHQAVQAWADSLDSDK
PLSLPL

Nucleotide

• Length: 261 bp

>AT25332 NC_015591:c298446-298186 [Sinorhizobium meliloti AK83]
ATGGAAACGAAGGTGCTGACGGCGCACGTACCACTGCCGCTCGCCGAGAAAGTCGATCAGATCGCGGCCCGGCTTGAGCG
CTCGCGCGGGTGGATCGTCAAGCAGGCGCTGACCGCCTGGGTCGATCAGGAAGAGGAGCGCCGACGCTTGACGCTGGAAG
CGCTTGCCGACGTCGACGCTGGCCGCGTTATCGATCACCAGGCCGTCCAGGCCTGGGCCGACAGTCTTGATAGTGACAAG
CCGCTCTCCTTGCCGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N8JT21