Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 119534..120059 Replicon plasmid pS234-2
Accession NZ_CP102188
Organism Klebsiella pneumoniae strain S234

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag NQZ63_RS28640 Protein ID WP_001159871.1
Coordinates 119534..119839 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag NQZ63_RS28645 Protein ID WP_000813634.1
Coordinates 119841..120059 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQZ63_RS28605 (115288) 115288..115932 + 645 WP_000633913.1 division plane positioning ATPase MipZ -
NQZ63_RS28610 (115926) 115926..116201 + 276 WP_000239529.1 hypothetical protein -
NQZ63_RS28615 (116393) 116393..116819 - 427 Protein_136 site-specific integrase -
NQZ63_RS28620 (116999) 116999..117589 + 591 Protein_137 colicin-B -
NQZ63_RS28630 (118358) 118358..118552 - 195 Protein_139 colicin M immunity protein -
NQZ63_RS28635 (118724) 118724..119533 - 810 WP_000016963.1 site-specific integrase -
NQZ63_RS28640 (119534) 119534..119839 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
NQZ63_RS28645 (119841) 119841..120059 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NQZ63_RS28650 (120618) 120618..121544 + 927 WP_001290414.1 hypothetical protein -
NQZ63_RS28655 (121594) 121594..121848 + 255 WP_000678528.1 hypothetical protein -
NQZ63_RS28660 (122346) 122346..122690 + 345 WP_000792769.1 hypothetical protein -
NQZ63_RS28665 (122864) 122864..123865 - 1002 WP_000785695.1 DUF4238 domain-containing protein -
NQZ63_RS28670 (124014) 124014..124253 - 240 Protein_147 hypothetical protein -
NQZ63_RS28675 (124210) 124210..124440 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
NQZ63_RS28680 (124437) 124437..124853 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA2 / dfrA14 / sul2 / qnrS1 / blaLAP-2 / blaKPC-2 / blaSHV-12 / tet(A) - 1..146760 146760
- flank IS/Tn - - 117605..118108 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T253316 WP_001159871.1 NZ_CP102188:c119839-119534 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT253316 WP_000813634.1 NZ_CP102188:c120059-119841 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References