Detailed information of TA system TA025331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3387370..3388031
• Replicon: chromosome
• Accession: NC_015590
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: SINME_RS16480
• Protein ID: WP_010970565.1
• Coordinates: 3387370..3387774 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92KY4
• Locus tag: SINME_RS16485
• Protein ID: WP_010970566.1
• Coordinates: 3387771..3388031 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS16465	3382913..3384025	+	1113	WP_003535893.1	3-isopropylmalate dehydrogenase	-
SINME_RS16470	3384154..3384861	+	708	WP_010970563.1	phosphatase PAP2 family protein	-
SINME_RS16475	3384962..3386851	-	1890	WP_013844938.1	MHS family MFS transporter	-
SINME_RS16480	3387370..3387774	-	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
SINME_RS16485	3387771..3388031	-	261	WP_010970566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SINME_RS35365	3388160..3388330	+	171	WP_010970567.1	hypothetical protein	-
SINME_RS16490	3388444..3389478	+	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
SINME_RS16495	3389651..3390472	+	822	WP_013844939.1	lytic transglycosylase family protein	-
SINME_RS16500	3390819..3391502	-	684	WP_010970569.1	carbonic anhydrase	-
SINME_RS16505	3391676..3392557	-	882	WP_010970570.1	pyridoxal kinase PdxY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T25331 WP_010970565.1 NC_015590:c3387774-3387370 [Sinorhizobium meliloti AK83]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T25331 NC_015590:c3387774-3387370 [Sinorhizobium meliloti AK83]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.16 Da
• Isoelectric Point: 5.1253

>AT25331 WP_010970566.1 NC_015590:c3388031-3387771 [Sinorhizobium meliloti AK83]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Nucleotide

• Length: 261 bp

>AT25331 NC_015590:c3388031-3387771 [Sinorhizobium meliloti AK83]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: Q92KY4