Detailed information of TA system TA025330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2749192..2749823
• Replicon: chromosome
• Accession: NC_015590
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SINME_RS13630
• Protein ID: WP_013844828.1
• Coordinates: 2749192..2749590 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: SINME_RS13635
• Protein ID: WP_010970129.1
• Coordinates: 2749590..2749823 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS13595	2744274..2744576	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SINME_RS13600	2744573..2744848	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
SINME_RS35260	2745134..2745283	+	150	WP_010970124.1	hypothetical protein	-
SINME_RS13610	2745280..2746215	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
SINME_RS13615	2746279..2747232	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
SINME_RS13620	2747477..2748919	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
SINME_RS13625	2748919..2749158	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
SINME_RS13630	2749192..2749590	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
SINME_RS13635	2749590..2749823	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SINME_RS13640	2749932..2751077	-	1146	WP_010970130.1	GTP-binding protein	-
SINME_RS13645	2751091..2752173	-	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
SINME_RS13650	2752425..2753588	+	1164	WP_010970132.1	amidohydrolase	-
SINME_RS13655	2753611..2754075	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T25330 WP_013844828.1 NC_015590:c2749590-2749192 [Sinorhizobium meliloti AK83]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T25330 NC_015590:c2749590-2749192 [Sinorhizobium meliloti AK83]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT25330 WP_010970129.1 NC_015590:c2749823-2749590 [Sinorhizobium meliloti AK83]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT25330 NC_015590:c2749823-2749590 [Sinorhizobium meliloti AK83]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9