25329

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacT-ataR/DUF1778(antitoxin)
Location 2701643..2702409 Replicon chromosome
Accession NC_015590
Organism Sinorhizobium meliloti AK83

Toxin (Protein)


Gene name tacT Uniprot ID H0G6D3
Locus tag SINME_RS13365 Protein ID WP_003533488.1
Coordinates 2701927..2702409 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID H0G6D4
Locus tag SINME_RS13360 Protein ID WP_003533490.1
Coordinates 2701643..2701927 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SINME_RS13345 2697113..2698501 + 1389 WP_003533497.1 lipopolysaccharide biosynthesis protein -
SINME_RS13350 2698505..2699776 + 1272 WP_010970087.1 GNAT family N-acetyltransferase -
SINME_RS13355 2699792..2701438 - 1647 WP_010970088.1 AMP-binding protein -
SINME_RS13360 2701643..2701927 + 285 WP_003533490.1 DUF1778 domain-containing protein Antitoxin
SINME_RS13365 2701927..2702409 + 483 WP_003533488.1 GNAT family N-acetyltransferase Toxin
SINME_RS13375 2702874..2703383 + 510 WP_013844819.1 disulfide bond formation protein B -
SINME_RS13380 2703415..2703972 - 558 WP_003533486.1 HNH endonuclease -
SINME_RS13385 2704078..2704725 - 648 WP_010970091.1 DNA-3-methyladenine glycosylase -
SINME_RS13390 2704878..2705774 + 897 WP_010970092.1 tRNA glutamyl-Q(34) synthetase GluQRS -
SINME_RS13395 2705793..2706668 - 876 WP_010970093.1 YihY/virulence factor BrkB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-142)

Antitoxin

Vibrio phage ICP1, Orf50

(1-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17463.88 Da        Isoelectric Point: 7.3827

>T25329 WP_003533488.1 NC_015590:2701927-2702409 [Sinorhizobium meliloti AK83]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Download         Length: 483 bp

>T25329 NC_015590:2701927-2702409 [Sinorhizobium meliloti AK83]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10771.12 Da        Isoelectric Point: 7.3175

>AT25329 WP_003533490.1 NC_015590:2701643-2701927 [Sinorhizobium meliloti AK83]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Download         Length: 285 bp

>AT25329 NC_015590:2701643-2701927 [Sinorhizobium meliloti AK83]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G6D3

Antitoxin

Source ID Structure
AlphaFold DB H0G6D4

References