253283

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5877769..5879498 Replicon chromosome
Accession NZ_CP102177
Organism Pseudomonas corrugata strain B21-055

Toxin (Protein)


Gene name pmenT Uniprot ID A0A8B6UP12
Locus tag LOY64_RS26200 Protein ID WP_055137583.1
Coordinates 5877769..5878413 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A8B6UP13
Locus tag LOY64_RS26205 Protein ID WP_055137582.1
Coordinates 5878410..5879498 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY64_RS26175 (LOY64_26190) 5873524..5874312 + 789 Protein_5157 IS256 family transposase -
LOY64_RS26180 (LOY64_26195) 5874489..5876003 + 1515 WP_053192610.1 IS21 family transposase -
LOY64_RS26185 (LOY64_26200) 5876024..5876779 + 756 WP_055137146.1 IS21-like element ISPsy14 family helper ATPase IstB -
LOY64_RS26190 (LOY64_26205) 5876885..5877358 + 474 Protein_5160 transposase -
LOY64_RS26195 (LOY64_26210) 5877439..5877768 + 330 WP_244251413.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
LOY64_RS26200 (LOY64_26215) 5877769..5878413 + 645 WP_055137583.1 DUF4433 domain-containing protein Toxin
LOY64_RS26205 (LOY64_26220) 5878410..5879498 + 1089 WP_055137582.1 macro domain-containing protein Antitoxin
LOY64_RS26210 (LOY64_26225) 5879690..5880049 - 360 WP_055137581.1 DUF6124 family protein -
LOY64_RS26215 (LOY64_26230) 5880532..5880990 - 459 WP_055137580.1 GNAT family N-acetyltransferase -
LOY64_RS26220 (LOY64_26235) 5881277..5881492 + 216 WP_024780087.1 DUF1737 domain-containing protein -
LOY64_RS26225 (LOY64_26240) 5881865..5882761 + 897 WP_265068306.1 HTH-type transcriptional regulator ArgP -
LOY64_RS26230 (LOY64_26245) 5882963..5883418 + 456 WP_175386570.1 hypothetical protein -
LOY64_RS26235 (LOY64_26250) 5883393..5883908 + 516 WP_024780084.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5871732..5883908 12176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24929.51 Da        Isoelectric Point: 8.4285

>T253283 WP_055137583.1 NZ_CP102177:5877769-5878413 [Pseudomonas corrugata]
MIYSRLNPEKALIWRIVHRDNLPWILDNGLHCANSTVRSSQYVNIGNADLIDKRRTRHVPIAPGGVLSDYVPFYFTPFSV
MMKNIHSGRSVQQRSNDEILILVSSLYLVEQMGFPFVYTNAHAYPDWTDYYSDLANLDQIDWSIIQRRDFKREPDDPRKM
ERYQAEALIHNHLPITGLLGIMCYTDAMKKRIDRDVAARGLTLSVHARPGWYFQ

Download         Length: 645 bp

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40827.81 Da        Isoelectric Point: 6.4007

>AT253283 WP_055137582.1 NZ_CP102177:5878410-5879498 [Pseudomonas corrugata]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAENYRLYSAACKAREVETGKVHVTAVNELEGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLIENDVKSVAVPPLGAGNGGLKWPEVREQIVEILSDLDVDVLVFEPSNQYLNISKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEQLPNTENPLNLRFVAHKYGPYANRLEHLLNNLDGSYLH
CDKRISDAGISDVIWFDEGRKAFLQAYLQTEAKEYTRALERTAELIDGFESPFGMELLATVDWLLSQDGISPTVPAVREA
LKHWDGGAGAAARKSKLFDDQALDIALKRLTSSSFRPEVATC

Download         Length: 1089 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6UP12

Antitoxin

Source ID Structure
AlphaFold DB A0A8B6UP13

References