Detailed information of TA system TA253280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5377929..5378451
• Replicon: chromosome
• Accession: NZ_CP102176
• Organism: Pseudomonas mediterranea strain B21-060

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LOY71_RS23340
• Protein ID: WP_159286032.1
• Coordinates: 5378158..5378451 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6I6QD85
• Locus tag: LOY71_RS23335
• Protein ID: WP_047702740.1
• Coordinates: 5377929..5378168 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY71_RS23310 (LOY71_23315)	5373210..5374547	+	1338	WP_159286031.1	phosphatase PAP2/dual specificity phosphatase family protein	-
LOY71_RS23315 (LOY71_23320)	5374537..5374986	+	450	WP_047702736.1	hypothetical protein	-
LOY71_RS23320 (LOY71_23325)	5375208..5375828	+	621	WP_047702737.1	lysophospholipid acyltransferase family protein	-
LOY71_RS23325 (LOY71_23330)	5375828..5376760	+	933	WP_047702738.1	phosphatidate cytidylyltransferase	-
LOY71_RS23330 (LOY71_23335)	5376821..5377711	-	891	WP_047702739.1	MBL fold metallo-hydrolase	-
LOY71_RS23335 (LOY71_23340)	5377929..5378168	+	240	WP_047702740.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LOY71_RS23340 (LOY71_23345)	5378158..5378451	+	294	WP_159286032.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY71_RS23345 (LOY71_23350)	5378508..5379350	-	843	WP_041021241.1	DUF2135 domain-containing protein	-
LOY71_RS23350 (LOY71_23355)	5379354..5380973	-	1620	WP_047702742.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11618.43 Da
• Isoelectric Point: 9.7094

>T253280 WP_159286032.1 NZ_CP102176:5378158-5378451 [Pseudomonas mediterranea]
MTYSLEFDRRALKEWNKLGDTLRQQFKKKLIEVLENPRIEANRLRQLPDCYKLKLRSAGYRLIYQVIDQEVVVFVVAIGK
REREAVYQDVQERISSD

Nucleotide

• Length: 294 bp

>T253280 NZ_CP102176:5378158-5378451 [Pseudomonas mediterranea]
ATGACCTATAGCCTCGAGTTTGATCGACGTGCATTAAAAGAATGGAACAAGCTAGGCGATACACTGCGACAACAGTTCAA
AAAGAAACTGATCGAGGTACTTGAAAACCCTCGCATCGAAGCCAATCGACTTAGACAACTTCCCGACTGCTACAAGTTGA
AATTGCGAAGCGCCGGGTATCGCCTGATCTACCAAGTTATTGATCAGGAAGTTGTGGTGTTCGTTGTTGCGATAGGAAAA
CGCGAGCGTGAAGCAGTTTATCAAGATGTGCAGGAGCGCATCTCATCCGATTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8896.21 Da
• Isoelectric Point: 4.6017

>AT253280 WP_047702740.1 NZ_CP102176:5377929-5378168 [Pseudomonas mediterranea]
MASINIRIDDDLKQRSFAELEKLGVTPSELLRQTLQYVAERGKLPFKAALLSEEDEALIAVVTERLSNPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT253280 NZ_CP102176:5377929-5378168 [Pseudomonas mediterranea]
ATGGCTTCTATCAACATTCGCATCGACGACGACCTGAAGCAGCGATCATTCGCCGAGCTGGAGAAGCTCGGCGTCACACC
TTCCGAGTTGCTGCGTCAGACACTTCAGTACGTAGCAGAGCGAGGCAAGCTGCCTTTTAAAGCAGCTCTGCTCAGTGAAG
AAGACGAAGCACTCATTGCGGTGGTAACGGAGCGTCTTTCCAACCCTCAACGAGTGAAGGTCAGCCTGGATGACCTATAG

Structures

Antitoxin

• AlphaFold DB: A0A6I6QD85