Detailed information of TA system TA025327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2013234..2013888
• Replicon: chromosome
• Accession: NC_015590
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: SINME_RS09995
• Protein ID: WP_003530010.1
• Coordinates: 2013234..2013422 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: SINME_RS10000
• Protein ID: WP_010969659.1
• Coordinates: 2013427..2013888 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS09980	2009800..2011110	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
SINME_RS09985	2011135..2012106	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
SINME_RS09990	2012287..2013057	-	771	WP_010969658.1	hypothetical protein	-
SINME_RS09995	2013234..2013422	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
SINME_RS10000	2013427..2013888	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SINME_RS10010	2014207..2015124	-	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
SINME_RS10015	2015301..2016050	-	750	WP_010969660.1	NUDIX hydrolase	-
SINME_RS10020	2016053..2017159	-	1107	WP_003530001.1	endonuclease	-
SINME_RS10025	2017310..2017753	-	444	WP_003529999.1	TIGR02594 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T25327 WP_003530010.1 NC_015590:2013234-2013422 [Sinorhizobium meliloti AK83]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T25327 NC_015590:2013234-2013422 [Sinorhizobium meliloti AK83]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT25327 WP_010969659.1 NC_015590:2013427-2013888 [Sinorhizobium meliloti AK83]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT25327 NC_015590:2013427-2013888 [Sinorhizobium meliloti AK83]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0