Detailed information of TA system TA025325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 530614..531254
• Replicon: chromosome
• Accession: NC_015590
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H0G6N1
• Locus tag: SINME_RS02540
• Protein ID: WP_003533691.1
• Coordinates: 530614..531000 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G6N0
• Locus tag: SINME_RS02545
• Protein ID: WP_003533689.1
• Coordinates: 531000..531254 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS02515	526078..526557	+	480	WP_003537307.1	GNAT family N-acetyltransferase	-
SINME_RS02520	526601..527050	-	450	WP_003537305.1	nucleoside deaminase	-
SINME_RS02525	527125..528846	+	1722	WP_013844081.1	pseudouridine synthase	-
SINME_RS02530	528853..529413	+	561	WP_010968831.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
SINME_RS02535	529486..530385	+	900	WP_010968832.1	patatin-like phospholipase family protein	-
SINME_RS02540	530614..531000	-	387	WP_003533691.1	type II toxin-antitoxin system VapC family toxin	Toxin
SINME_RS02545	531000..531254	-	255	WP_003533689.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SINME_RS02550	531393..533225	-	1833	WP_013844083.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
SINME_RS02555	533377..534723	+	1347	WP_010968834.1	TldD/PmbA family protein	-
SINME_RS02560	534832..535650	+	819	WP_010968835.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
SINME_RS02565	535711..535947	+	237	WP_003533684.1	DUF4170 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13904.77 Da
• Isoelectric Point: 4.5716

>T25325 WP_003533691.1 NC_015590:c531000-530614 [Sinorhizobium meliloti AK83]
MVIDTSAIAAIAFNEQEAGSFREKIADDPVRLISAATALEAAMVIETRLGEAAGAELDLWLYKANVEIVAVTAEHMDQAR
RAWRRFGKGRHPAGLNFGDCFSYALAFLTNEPLLFKGSDFSQTDIPAA

Nucleotide

• Length: 387 bp

>T25325 NC_015590:c531000-530614 [Sinorhizobium meliloti AK83]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCGGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTCTTCAAGGGAAGCGATTTCTCGCAAACCGATATCCCGGCGGCATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9472.63 Da
• Isoelectric Point: 4.8660

>AT25325 WP_003533689.1 NC_015590:c531254-531000 [Sinorhizobium meliloti AK83]
MALSIKDTETEQLARTLARRTGESITLATKRALEERLRRTDTAARRAALLEDLEAIQRRWNAMPVLDSRSSDDIIGYDED
GLPS

Nucleotide

• Length: 255 bp

>AT25325 NC_015590:c531254-531000 [Sinorhizobium meliloti AK83]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGCACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AAGCCATTCAACGCCGCTGGAATGCCATGCCCGTTCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Toxin

• AlphaFold DB: H0G6N1

Antitoxin

• AlphaFold DB: H0G6N0