Detailed information of TA system TA025324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 320167..320744
• Replicon: chromosome
• Accession: NC_015590
• Organism: Sinorhizobium meliloti AK83

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: SINME_RS34585
• Protein ID: WP_010968707.1
• Coordinates: 320167..320430 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: SINME_RS01525
• Protein ID: WP_010968708.1
• Coordinates: 320427..320744 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SINME_RS01485	315496..315936	+	441	WP_010968702.1	DUF2948 family protein	-
SINME_RS01490	316001..317305	+	1305	WP_010968703.1	histidinol dehydrogenase	-
SINME_RS01495	317302..317778	+	477	WP_010968704.1	UPF0262 family protein	-
SINME_RS01500	317792..318271	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
SINME_RS01505	318429..318647	+	219	WP_004435948.1	translation initiation factor IF-1	-
SINME_RS01510	318745..319365	+	621	WP_004435951.1	Maf-like protein	-
SINME_RS01515	319370..319582	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
SINME_RS34585	320167..320430	+	264	WP_010968707.1	BrnT family toxin	Toxin
SINME_RS01525	320427..320744	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
SINME_RS01530	320947..321411	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
SINME_RS34590	321408..321596	-	189	WP_013844020.1	hypothetical protein	-
SINME_RS01535	321916..323016	+	1101	WP_010968710.1	hypothetical protein	-
SINME_RS01540	323003..324085	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
SINME_RS01545	324288..324599	+	312	WP_004435964.1	hypothetical protein	-
SINME_RS01555	324879..325268	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	264479..321596	57117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T25324 WP_010968707.1 NC_015590:320167-320430 [Sinorhizobium meliloti AK83]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T25324 NC_015590:320167-320430 [Sinorhizobium meliloti AK83]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT25324 WP_010968708.1 NC_015590:320427-320744 [Sinorhizobium meliloti AK83]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT25324 NC_015590:320427-320744 [Sinorhizobium meliloti AK83]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5