25323

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 89251..89816 Replicon chromosome
Accession NC_015590
Organism Sinorhizobium meliloti AK83

Toxin (Protein)


Gene name brnT Uniprot ID A0A843ZV60
Locus tag SINME_RS00415 Protein ID WP_013843871.1
Coordinates 89571..89816 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A843ZXZ1
Locus tag SINME_RS00410 Protein ID WP_013843870.1
Coordinates 89251..89574 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SINME_RS00390 85114..85914 + 801 WP_013843867.1 TVP38/TMEM64 family protein -
SINME_RS00395 85937..87358 + 1422 WP_013843868.1 NAD(P)/FAD-dependent oxidoreductase -
SINME_RS00400 87433..88953 + 1521 WP_013843869.1 HAMP domain-containing histidine kinase -
SINME_RS00410 89251..89574 - 324 WP_013843870.1 BrnA antitoxin family protein Antitoxin
SINME_RS00415 89571..89816 - 246 WP_013843871.1 BrnT family toxin Toxin
SINME_RS00420 90253..90966 + 714 WP_013843872.1 replicative DNA helicase -
SINME_RS00425 91151..91846 - 696 WP_013843873.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
SINME_RS00430 92038..92526 + 489 WP_027989489.1 RidA family protein -
SINME_RS00435 92735..93352 + 618 WP_003531558.1 hypothetical protein -
SINME_RS00440 93440..94585 + 1146 WP_013843875.1 alpha-hydroxy-acid oxidizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-77)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-101)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9004.50 Da        Isoelectric Point: 9.7323

>T25323 WP_013843871.1 NC_015590:c89816-89571 [Sinorhizobium meliloti AK83]
MKITYDETKRQINIGKHGYDIAELDMEFFASSVVVPAKEGRFMAIGVLRNGVIAVVFARLGSEGISIISMRDASRKERSL
L

Download         Length: 246 bp

>T25323 NC_015590:c89816-89571 [Sinorhizobium meliloti AK83]
ATGAAGATCACCTATGACGAAACAAAGCGCCAGATCAACATAGGAAAGCACGGCTATGATATCGCCGAACTCGACATGGA
GTTCTTCGCTTCGTCTGTGGTCGTCCCAGCCAAGGAAGGCCGCTTCATGGCTATCGGCGTTTTGCGCAATGGCGTTATCG
CCGTAGTCTTCGCCCGGCTTGGCTCGGAAGGCATTTCGATTATCTCCATGCGTGACGCCAGCAGGAAGGAAAGGAGCTTA
TTATGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11977.52 Da        Isoelectric Point: 10.0454

>AT25323 WP_013843870.1 NC_015590:c89574-89251 [Sinorhizobium meliloti AK83]
MTSLSRRIRHISDKEEAAIQRKIDSDPDAPEATDEQLAKAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDVIE
HYKATGKGWQSRMNDDLRKAAGLKKAG

Download         Length: 324 bp

>AT25323 NC_015590:c89574-89251 [Sinorhizobium meliloti AK83]
ATGACCAGCCTATCCCGCCGCATCCGCCATATCTCCGACAAGGAGGAGGCAGCTATCCAACGCAAGATAGATAGTGACCC
AGACGCTCCTGAGGCCACCGACGAACAACTAGCCAAAGCTAAGCCATTCGCCGAAGCCTTCCCAGACCTGGCCGAGAGCA
TCAAGCGCGCCAGGGGCCGTCCAGCCGTTGAGAACCCTAGGCAGCAGATATCCATTCGCCTCGATCCGGATGTCATCGAG
CACTACAAGGCAACCGGCAAAGGCTGGCAGAGCCGGATGAATGACGACCTTAGAAAGGCTGCCGGATTGAAGAAGGCAGG
TTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A843ZV60

Antitoxin

Source ID Structure
AlphaFold DB A0A843ZXZ1

References