Detailed information of TA system TA025321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2021636..2022222
• Replicon: chromosome
• Accession: NC_015588
• Organism: Isoptericola variabilis 225

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F6FX20
• Locus tag: ISOVA_RS09445
• Protein ID: WP_013839012.1
• Coordinates: 2021878..2022222 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F6FX19
• Locus tag: ISOVA_RS09440
• Protein ID: WP_013839011.1
• Coordinates: 2021636..2021884 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISOVA_RS09415	2017251..2017448	-	198	WP_013839006.1	hypothetical protein	-
ISOVA_RS09420	2017468..2018256	+	789	WP_049788302.1	ROK family protein	-
ISOVA_RS09425	2018331..2018654	-	324	WP_013839008.1	STAS domain-containing protein	-
ISOVA_RS09430	2018904..2020976	+	2073	WP_013839009.1	elongation factor G	-
ISOVA_RS09435	2021016..2021597	+	582	WP_013839010.1	MBL fold metallo-hydrolase	-
ISOVA_RS09440	2021636..2021884	+	249	WP_013839011.1	ribbon-helix-helix protein, CopG family	Antitoxin
ISOVA_RS09445	2021878..2022222	+	345	WP_013839012.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISOVA_RS09450	2022708..2023090	-	383	Protein_1866	tautomerase family protein	-
ISOVA_RS09455	2023169..2024002	+	834	WP_013839013.1	peroxide stress protein YaaA	-
ISOVA_RS15520	2024110..2024373	+	264	WP_013839014.1	TfoX/Sxy family protein	-
ISOVA_RS09465	2024383..2025561	-	1179	WP_013839015.1	bifunctional RNase H/acid phosphatase	-
ISOVA_RS09470	2025561..2026307	-	747	WP_013839016.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12229.17 Da
• Isoelectric Point: 8.0218

>T25321 WP_013839012.1 NC_015588:2021878-2022222 [Isoptericola variabilis 225]
VVIRRGEICWVDFGEPLGSAPAKRRPAVVVQSEQYNRSRISTVVVLPITSNTALARHPGNVFLPALASGLPKDSVVNVSQ
PMTVDRADIEATGVMLPGNLMEAVEAGLRRVIDL

Nucleotide

• Length: 345 bp

>T25321 NC_015588:2021878-2022222 [Isoptericola variabilis 225]
GTGGTGATCCGGCGCGGGGAGATCTGCTGGGTCGACTTCGGCGAACCTCTGGGCAGCGCTCCGGCCAAGCGTCGTCCGGC
AGTCGTCGTTCAGTCGGAGCAGTACAACAGATCCCGCATCTCCACGGTCGTCGTCCTGCCCATCACGTCCAATACCGCCC
TTGCCCGGCACCCAGGAAACGTGTTCCTCCCGGCGCTCGCCTCGGGCCTGCCGAAGGACTCGGTCGTCAACGTGTCTCAG
CCGATGACCGTGGACCGGGCCGACATCGAAGCGACCGGGGTCATGCTCCCGGGGAACCTCATGGAAGCCGTGGAGGCAGG
CCTGCGTCGCGTCATCGACCTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9105.91 Da
• Isoelectric Point: 4.4325

>AT25321 WP_013839011.1 NC_015588:2021636-2021884 [Isoptericola variabilis 225]
MKTAISLPDSAAEHFDRVAKKHGMTRSEFYRRAAEHYVEELDGADLTAQIDDAIDDVGQPGDETAELRRAANTRLTEASG
EW

Nucleotide

• Length: 249 bp

>AT25321 NC_015588:2021636-2021884 [Isoptericola variabilis 225]
ATGAAGACGGCGATCAGCCTCCCGGACAGCGCGGCGGAGCATTTCGACCGCGTCGCCAAGAAGCACGGCATGACCCGGTC
GGAGTTCTACCGGCGTGCCGCCGAGCATTACGTCGAGGAGCTGGACGGCGCAGACCTGACCGCGCAGATCGACGACGCGA
TCGATGACGTCGGCCAGCCCGGCGACGAGACCGCCGAGCTCCGCCGTGCTGCAAACACCCGCCTGACCGAGGCGTCGGGC
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: F6FX20

Antitoxin

• AlphaFold DB: F6FX19