Detailed information of TA system TA025319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1053845..1054467
• Replicon: chromosome
• Accession: NC_015581
• Organism: Thioalkalimicrobium cyclicum ALM1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: THICY_RS04640
• Protein ID: WP_013835454.1
• Coordinates: 1053845..1054243 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: THICY_RS04645
• Protein ID: WP_157862753.1
• Coordinates: 1054240..1054467 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THICY_RS04585	1049219..1049434	-	216	WP_013835441.1	twitching motility protein PilT	-
THICY_RS04590	1049421..1049660	-	240	WP_013835442.1	hypothetical protein	-
THICY_RS04595	1049861..1050124	-	264	WP_013835444.1	Txe/YoeB family addiction module toxin	-
THICY_RS04600	1050129..1050371	-	243	WP_013835445.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
THICY_RS04605	1050727..1050945	-	219	WP_013835447.1	hypothetical protein	-
THICY_RS04610	1050929..1051207	-	279	WP_013835448.1	BrnT family toxin	-
THICY_RS04615	1051604..1051987	-	384	WP_013835449.1	type II toxin-antitoxin system VapC family toxin	-
THICY_RS04620	1051991..1052221	-	231	WP_013835450.1	hypothetical protein	-
THICY_RS04625	1052392..1052676	-	285	WP_013835451.1	GIY-YIG nuclease family protein	-
THICY_RS04630	1053134..1053469	-	336	WP_157862721.1	PIN domain-containing protein	-
THICY_RS04635	1053505..1053735	-	231	WP_013835453.1	hypothetical protein	-
THICY_RS04640	1053845..1054243	-	399	WP_013835454.1	tRNA(fMet)-specific endonuclease VapC	Toxin
THICY_RS04645	1054240..1054467	-	228	WP_157862753.1	antitoxin	Antitoxin
THICY_RS04650	1054738..1055829	-	1092	WP_013835456.1	UDP-glucose 4-epimerase GalE	-
THICY_RS04655	1055968..1057308	-	1341	WP_013835457.1	exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase	-
THICY_RS04660	1057395..1057865	-	471	WP_013835458.1	glycerol-3-phosphate cytidylyltransferase	-
THICY_RS04665	1057935..1058933	-	999	WP_013835459.1	UDP-glucose 4-epimerase GalE	-
THICY_RS04670	1059023..1059211	-	189	Protein_904	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-116)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14939.15 Da
• Isoelectric Point: 6.8706

>T25319 WP_013835454.1 NC_015581:c1054243-1053845 [Thiomicrospira cyclica ALM1]
MIKYLLDTNILIYTLKNRPRQVRDAFNSHVTQMAISTITVGELIYGAERSNQPERNLADIEGLIARLDVLDFDSHAATHF
GQIRAELYAKGQPIGPYDMMIAGHARSRGLILVSNNLCEFERVPGLRLENWV

Nucleotide

• Length: 399 bp

>T25319 NC_015581:c1054243-1053845 [Thiomicrospira cyclica ALM1]
ATGATCAAGTATTTGCTCGATACCAATATTCTGATTTATACCTTAAAAAACCGACCTAGGCAGGTCCGTGATGCTTTTAA
TAGTCATGTGACGCAGATGGCGATTTCGACGATTACAGTTGGCGAATTGATTTACGGTGCCGAACGATCTAACCAACCTG
AGCGGAATCTTGCCGATATTGAAGGTTTGATCGCGCGTTTGGATGTGCTGGATTTTGATAGCCATGCCGCGACTCATTTT
GGTCAAATTCGCGCAGAACTTTATGCAAAAGGTCAACCAATAGGCCCTTATGACATGATGATTGCCGGACACGCTCGAAG
CCGAGGTCTTATTTTGGTGAGCAATAACCTGTGTGAATTTGAACGCGTTCCGGGTTTAAGGCTCGAGAATTGGGTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8519.59 Da
• Isoelectric Point: 4.3148

>AT25319 WP_157862753.1 NC_015581:c1054467-1054240 [Thiomicrospira cyclica ALM1]
ITTTLFKNNQSQAVRLPKSVALDESIKQVVITAIGKTRIITPLEQTWDDWFDAVGVSDDFMNERAQEPDQLRESL

Nucleotide

• Length: 228 bp

>AT25319 NC_015581:c1054467-1054240 [Thiomicrospira cyclica ALM1]
ATTACAACAACCCTTTTTAAAAATAACCAATCTCAGGCCGTACGTTTGCCAAAATCGGTTGCCTTGGACGAAAGTATTAA
GCAGGTTGTCATTACCGCAATTGGCAAAACTCGCATTATCACCCCGCTTGAACAGACGTGGGATGATTGGTTTGATGCTG
TAGGTGTGAGCGATGATTTTATGAACGAGCGTGCGCAAGAGCCGGATCAGCTTCGCGAATCCTTATGA