Detailed information of TA system TA025316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-parD/RelE-CC2985
• Location: 3379499..3380061
• Replicon: chromosome
• Accession: NC_015580
• Organism: Novosphingobium sp. PP1Y

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G6EDG6
• Locus tag: PP1Y_RS21975
• Protein ID: WP_007013302.1
• Coordinates: 3379499..3379792 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F6IHT4
• Locus tag: PP1Y_RS21980
• Protein ID: WP_013834143.1
• Coordinates: 3379792..3380061 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP1Y_RS25890	3375009..3375323	+	315	WP_148275044.1	hypothetical protein	-
PP1Y_RS21940	3375482..3376462	-	981	WP_013834138.1	DUF2493 domain-containing protein	-
PP1Y_RS21950	3376873..3377115	-	243	WP_041559089.1	hypothetical protein	-
PP1Y_RS21955	3377184..3378182	-	999	WP_013834139.1	DUF2493 domain-containing protein	-
PP1Y_RS21960	3378278..3378739	-	462	WP_013834140.1	DUF736 domain-containing protein	-
PP1Y_RS21965	3378946..3379137	+	192	WP_041559090.1	hypothetical protein	-
PP1Y_RS21970	3379221..3379433	-	213	WP_041559091.1	hypothetical protein	-
PP1Y_RS21975	3379499..3379792	-	294	WP_007013302.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PP1Y_RS21980	3379792..3380061	-	270	WP_013834143.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
PP1Y_RS21985	3380427..3381170	-	744	WP_148275144.1	hypothetical protein	-
PP1Y_RS21990	3381359..3381646	-	288	WP_041559092.1	hypothetical protein	-
PP1Y_RS21995	3381720..3382499	-	780	WP_013834145.1	hypothetical protein	-
PP1Y_RS22000	3382589..3384040	+	1452	WP_038577292.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

• Antitoxin ParD (3-67)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10792.30 Da
• Isoelectric Point: 6.2147

>T25316 WP_007013302.1 NC_015580:c3379792-3379499 [Novosphingobium sp. PP1Y]
MHLQFSPAAEADLVDIAAFIARDNVPRAFTFVDELEAACARLVDFPESGSARFDLRPGLRSRPHGSYVIFYSTLDRVIRI
ERILHGARDVGAILFSS

Nucleotide

• Length: 294 bp

>T25316 NC_015580:c3379792-3379499 [Novosphingobium sp. PP1Y]
ATGCATCTGCAGTTTTCGCCCGCGGCGGAAGCAGACCTTGTCGATATTGCCGCCTTCATCGCTCGCGACAATGTTCCGCG
GGCTTTCACCTTTGTTGACGAACTTGAAGCGGCATGTGCCAGACTTGTCGATTTCCCCGAGTCCGGGAGTGCTCGTTTCG
ACCTTCGTCCCGGGCTGCGCTCCAGACCGCACGGTAGCTATGTGATCTTCTACAGCACCCTGGACAGGGTCATTCGTATC
GAGCGTATTCTGCATGGGGCCCGCGATGTCGGCGCGATCCTCTTTTCCAGTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9558.65 Da
• Isoelectric Point: 6.4653

>AT25316 WP_013834143.1 NC_015580:c3380061-3379792 [Novosphingobium sp. PP1Y]
MPSSFTLGAHFEGFIKSQVNSGRYASASEVVRDSLRLLEEQDAMRQARLDALRAEIEHGATSGPGIPAEQAFANARKRIA
DIAAAARDS

Nucleotide

• Length: 270 bp

>AT25316 NC_015580:c3380061-3379792 [Novosphingobium sp. PP1Y]
ATGCCTTCCAGCTTTACCCTGGGTGCCCATTTCGAGGGCTTCATCAAGAGCCAGGTGAACAGCGGTCGCTACGCGTCCGC
CAGCGAGGTCGTTCGCGATAGCCTCCGCTTGCTCGAGGAACAGGACGCAATGCGTCAGGCTCGGCTCGATGCGCTGCGCG
CCGAGATCGAGCACGGCGCTACCAGCGGTCCGGGCATTCCTGCTGAGCAGGCATTCGCGAACGCACGCAAGCGGATCGCT
GATATCGCCGCCGCCGCCAGGGATAGTTGA

Structures

Toxin

• AlphaFold DB: G6EDG6

Antitoxin

• AlphaFold DB: F6IHT4