Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 158501..159144 Replicon plasmid p5589-CTX-M-55
Accession NZ_CP102078
Organism Klebsiella pneumoniae strain 5589

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag NOV87_RS26490 Protein ID WP_001044768.1
Coordinates 158501..158917 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag NOV87_RS26495 Protein ID WP_001261287.1
Coordinates 158914..159144 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NOV87_RS26475 (NOV87_26475) 155000..155590 - 591 WP_000194575.1 hypothetical protein -
NOV87_RS26480 (NOV87_26480) 155590..155847 - 258 WP_000343085.1 hypothetical protein -
NOV87_RS26485 (NOV87_26485) 156201..158339 + 2139 WP_000350638.1 AAA family ATPase -
NOV87_RS26490 (NOV87_26490) 158501..158917 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
NOV87_RS26495 (NOV87_26495) 158914..159144 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NOV87_RS26500 (NOV87_26500) 159440..159730 + 291 WP_000111771.1 hypothetical protein -
NOV87_RS26505 (NOV87_26505) 159720..160619 + 900 WP_000963206.1 nucleotide-binding protein -
NOV87_RS26510 (NOV87_26510) 160669..162894 - 2226 WP_000698737.1 P-loop NTPase fold protein -
NOV87_RS26515 (NOV87_26515) 162896..163984 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib / fosA3 / blaTEM-1B / blaCTX-M-55 / mph(E) / msr(E) - 1..290720 290720


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T253157 WP_001044768.1 NZ_CP102078:c158917-158501 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT253157 WP_001261287.1 NZ_CP102078:c159144-158914 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References