Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5190177..5190802 Replicon chromosome
Accession NZ_CP102077
Organism Klebsiella pneumoniae strain 5589

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NOV87_RS25210 Protein ID WP_002882817.1
Coordinates 5190177..5190560 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NOV87_RS25215 Protein ID WP_004150355.1
Coordinates 5190560..5190802 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NOV87_RS25195 (5187543) 5187543..5188445 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NOV87_RS25200 (5188442) 5188442..5189077 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NOV87_RS25205 (5189074) 5189074..5190003 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NOV87_RS25210 (5190177) 5190177..5190560 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NOV87_RS25215 (5190560) 5190560..5190802 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NOV87_RS25220 (5191007) 5191007..5191924 + 918 WP_021466676.1 alpha/beta hydrolase -
NOV87_RS25225 (5191938) 5191938..5192879 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NOV87_RS25230 (5192924) 5192924..5193361 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NOV87_RS25235 (5193358) 5193358..5194218 - 861 WP_004146232.1 virulence factor BrkB family protein -
NOV87_RS25240 (5194212) 5194212..5194811 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T253155 WP_002882817.1 NZ_CP102077:c5190560-5190177 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT253155 WP_004150355.1 NZ_CP102077:c5190802-5190560 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References