Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 4484..5621 Replicon plasmid pJF3A-4253-4
Accession NZ_CP102073
Organism Enterococcus faecalis strain JF3A-4253

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag NP948_RS14830 Protein ID WP_256969069.1
Coordinates 4484..5347 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag NP948_RS14835 Protein ID WP_000301765.1
Coordinates 5349..5621 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP948_RS14815 1281..2255 - 975 WP_256969065.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
NP948_RS14820 2255..3487 - 1233 WP_256969066.1 DNA adenine methylase -
NP948_RS14825 3736..4420 + 685 WP_256969067.1 IS6 family transposase -
NP948_RS14830 4484..5347 - 864 WP_256969069.1 zeta toxin family protein Toxin
NP948_RS14835 5349..5621 - 273 WP_000301765.1 antitoxin Antitoxin
NP948_RS14840 5638..5853 - 216 WP_001835296.1 peptide-binding protein -
NP948_RS14845 5945..6841 - 897 WP_002387620.1 ParA family protein -
NP948_RS14850 6930..9074 - 2145 WP_256969070.1 type IA DNA topoisomerase -
NP948_RS14855 9074..9691 - 618 WP_128712832.1 recombinase family protein -
NP948_RS14860 9705..9875 - 171 WP_164742224.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid Cfr(D) / poxtA / fexA - 1..21806 21806
- flank IS/Tn - - 3959..4420 461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32440.98 Da        Isoelectric Point: 6.6610

>T253140 WP_256969069.1 NZ_CP102073:c5347-4484 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVIAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKIPKLPGL

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT253140 WP_000301765.1 NZ_CP102073:c5621-5349 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References