Detailed information of TA system TA025314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1734436..1734995
• Replicon: chromosome
• Accession: NC_015580
• Organism: Novosphingobium sp. PP1Y

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F6IM68
• Locus tag: PP1Y_RS14290
• Protein ID: WP_013832885.1
• Coordinates: 1734702..1734995 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F6IM67
• Locus tag: PP1Y_RS14285
• Protein ID: WP_013832884.1
• Coordinates: 1734436..1734705 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP1Y_RS14270	1731477..1732508	-	1032	WP_041558869.1	FecR domain-containing protein	-
PP1Y_RS14275	1732505..1733167	-	663	WP_148274976.1	RNA polymerase sigma factor	-
PP1Y_RS14280	1733442..1734365	+	924	WP_013832883.1	HEPN domain-containing protein	-
PP1Y_RS14285	1734436..1734705	+	270	WP_013832884.1	ribbon-helix-helix domain-containing protein	Antitoxin
PP1Y_RS14290	1734702..1734995	+	294	WP_013832885.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PP1Y_RS14295	1735017..1735427	-	411	WP_013832886.1	MarR family transcriptional regulator	-
PP1Y_RS14300	1735420..1736382	-	963	WP_148274977.1	phytanoyl-CoA dioxygenase family protein	-
PP1Y_RS14305	1736397..1737020	-	624	WP_148274978.1	GNAT family N-acetyltransferase	-
PP1Y_RS14310	1737074..1737841	-	768	WP_013832889.1	LuxR family transcriptional regulator	-
PP1Y_RS14315	1738123..1738320	-	198	WP_013832890.1	AlpA family transcriptional regulator	-
PP1Y_RS26030	1738686..1738973	+	288	WP_158511857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1699873..1740603	40730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11351.99 Da
• Isoelectric Point: 9.0225

>T25314 WP_013832885.1 NC_015580:1734702-1734995 [Novosphingobium sp. PP1Y]
MKIQWTSKASSDLVRLHEHLRPVAPDAAARIVQELARAPDRLLDYPRLGERLDAFDPREVRRIIVGNYEMRYEISQATIF
ILRLWHCRENRGTGPDN

Nucleotide

• Length: 294 bp

>T25314 NC_015580:1734702-1734995 [Novosphingobium sp. PP1Y]
ATGAAGATCCAGTGGACCAGCAAGGCCTCTTCCGACCTTGTTCGCCTTCATGAACATCTGAGGCCTGTCGCGCCCGATGC
CGCGGCGCGCATTGTCCAGGAATTGGCCCGTGCGCCGGACAGGTTGCTGGATTACCCCCGGCTCGGCGAGAGGCTGGATG
CCTTCGATCCTCGCGAGGTCCGCCGGATCATCGTGGGCAACTACGAAATGCGCTACGAAATTTCGCAGGCCACGATTTTC
ATTCTTCGACTTTGGCATTGCCGGGAAAATCGGGGCACCGGTCCGGATAACTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9868.20 Da
• Isoelectric Point: 4.7961

>AT25314 WP_013832884.1 NC_015580:1734436-1734705 [Novosphingobium sp. PP1Y]
MAAVTRVITAHVSADLADKVDVLAERMDRSRGWIMKQALTAWIDQEEERHRLTLEALHDVDAGNVIDHQSIVAWAESLST
KDPLAPPLA

Nucleotide

• Length: 270 bp

>AT25314 NC_015580:1734436-1734705 [Novosphingobium sp. PP1Y]
ATGGCAGCTGTTACCCGCGTCATCACCGCCCATGTGTCCGCCGACCTGGCGGACAAGGTCGATGTCCTGGCAGAACGGAT
GGACCGGTCGCGTGGCTGGATCATGAAGCAGGCCCTGACCGCCTGGATCGACCAGGAAGAGGAGCGGCACCGGCTTACGC
TTGAAGCGCTGCATGATGTCGATGCCGGCAATGTGATCGACCATCAGAGCATCGTCGCCTGGGCCGAGAGCCTTTCCACG
AAAGATCCGCTGGCCCCGCCCTTGGCATGA

Structures

Toxin

• AlphaFold DB: F6IM68

Antitoxin

• AlphaFold DB: F6IM67