Detailed information of TA system TA025312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 161547..162076
• Replicon: plasmid Lpl
• Accession: NC_015579
• Organism: Novosphingobium sp. PP1Y

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: F6ICF5
• Locus tag: PP1Y_RS01000
• Protein ID: WP_013831438.1
• Coordinates: 161547..161792 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: F6ICF6
• Locus tag: PP1Y_RS01005
• Protein ID: WP_013831439.1
• Coordinates: 161789..162076 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP1Y_RS00975	157280..159247	+	1968	WP_158511815.1	VWA domain-containing protein	-
PP1Y_RS00980	159259..159885	+	627	WP_013831435.1	pilus assembly protein	-
PP1Y_RS00985	159885..160535	+	651	WP_013831436.1	hypothetical protein	-
PP1Y_RS25090	160591..160950	+	360	WP_148274764.1	hypothetical protein	-
PP1Y_RS01000	161547..161792	+	246	WP_013831438.1	BrnT family toxin	Toxin
PP1Y_RS01005	161789..162076	+	288	WP_013831439.1	BrnA antitoxin family protein	Antitoxin
PP1Y_RS01010	162449..163303	-	855	WP_041558059.1	hypothetical protein	-
PP1Y_RS01015	163303..164946	-	1644	WP_013831441.1	ATP-binding protein	-
PP1Y_RS01020	165522..166766	-	1245	WP_013831442.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..192103	192103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-74)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (22-92)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8753.29 Da
• Isoelectric Point: 5.7551

>T25312 WP_013831438.1 NC_015579:161547-161792 [Novosphingobium sp. PP1Y]
MIIVWDEPKRQANLVKHGIDFADVGEAFFLSALVIPAKDGRFAAIGEMNGTITVIFAVLGTEGVSIISARPASIMERRLL
P

Nucleotide

• Length: 246 bp

>T25312 NC_015579:161547-161792 [Novosphingobium sp. PP1Y]
ATGATCATCGTATGGGATGAACCGAAACGGCAAGCGAACCTCGTCAAGCACGGGATCGACTTTGCCGATGTGGGGGAAGC
GTTCTTCCTCTCCGCTCTGGTTATCCCCGCGAAGGACGGACGCTTTGCCGCCATCGGTGAGATGAACGGCACGATCACGG
TGATTTTCGCTGTGCTGGGCACCGAAGGCGTCTCGATCATCTCTGCCCGCCCGGCCAGCATTATGGAACGGAGGCTCCTG
CCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10485.85 Da
• Isoelectric Point: 8.6239

>AT25312 WP_013831439.1 NC_015579:161789-162076 [Novosphingobium sp. PP1Y]
MTDPKHVAPGYTKADMDEVSNNPEWTAEDFARAKPFAEAFPDLAKTIRARGPQKAPKKVSTTLRLSPEVIEHFKSGGPGW
QSRIDAALKDWVAAH

Nucleotide

• Length: 288 bp

>AT25312 NC_015579:161789-162076 [Novosphingobium sp. PP1Y]
ATGACCGACCCGAAACACGTCGCTCCCGGATACACCAAGGCCGATATGGATGAGGTCAGTAATAACCCCGAATGGACAGC
CGAGGACTTCGCCAGGGCAAAGCCTTTCGCTGAGGCCTTCCCGGACCTCGCAAAGACGATTCGCGCACGCGGTCCGCAGA
AGGCCCCGAAAAAGGTCAGCACCACGCTGCGCCTTTCGCCCGAGGTGATCGAGCATTTCAAATCCGGCGGTCCCGGTTGG
CAGTCACGGATCGATGCTGCCCTCAAGGATTGGGTGGCCGCGCACTGA

Structures

Toxin

• AlphaFold DB: F6ICF5

Antitoxin

• AlphaFold DB: F6ICF6