Detailed information of TA system TA253119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 17756..18893
• Replicon: plasmid pJF3A-223-4
• Accession: NZ_CP102068
• Organism: Enterococcus faecalis strain JF3A-223

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: NP947_RS14475
• Protein ID: WP_256969069.1
• Coordinates: 18030..18893 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: NP947_RS14470
• Protein ID: WP_000301765.1
• Coordinates: 17756..18028 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NP947_RS14455	14615..16447	+	1833	WP_256969963.1	type IA DNA topoisomerase	-
NP947_RS14460	16536..17432	+	897	WP_002387620.1	ParA family protein	-
NP947_RS14465	17524..17739	+	216	WP_001835296.1	peptide-binding protein	-
NP947_RS14470	17756..18028	+	273	WP_000301765.1	antitoxin	Antitoxin
NP947_RS14475	18030..18893	+	864	WP_256969069.1	zeta toxin family protein	Toxin
NP947_RS14485	19890..21122	+	1233	WP_256969066.1	DNA adenine methylase	-
NP947_RS14490	21122..22096	+	975	WP_256969065.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	fexA / poxtA / Cfr(D)	-	1..26169	26169
	-	flank	IS/Tn	-	-	18957..19418	461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32440.98 Da
• Isoelectric Point: 6.6610

>T253119 WP_256969069.1 NZ_CP102068:18030-18893 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVIAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKIPKLPGL

Nucleotide

• Length: 864 bp

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT253119 WP_000301765.1 NZ_CP102068:17756-18028 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3