Detailed information of TA system TA025311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 90891..92382
• Replicon: plasmid Lpl
• Accession: NC_015579
• Organism: Novosphingobium sp. PP1Y

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: F6IC73
• Locus tag: PP1Y_RS00660
• Protein ID: WP_013831356.1
• Coordinates: 90891..92156 (-)
• Length: 422 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: PP1Y_RS00665
• Protein ID: WP_013831357.1
• Coordinates: 92146..92382 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP1Y_RS00645	86441..87634	+	1194	WP_013831353.1	AAA family ATPase	-
PP1Y_RS00650	87744..88817	+	1074	WP_013831354.1	ParB/RepB/Spo0J family partition protein	-
PP1Y_RS00655	88922..90199	+	1278	WP_013831355.1	replication initiator protein A	-
PP1Y_RS00660	90891..92156	-	1266	WP_013831356.1	type II toxin-antitoxin system HipA family toxin	Toxin
PP1Y_RS00665	92146..92382	-	237	WP_013831357.1	helix-turn-helix transcriptional regulator	Antitoxin
PP1Y_RS00670	92999..94036	+	1038	WP_013831359.1	tyrosine-type recombinase/integrase	-
PP1Y_RS00675	94070..94312	+	243	WP_041558020.1	hypothetical protein	-
PP1Y_RS00680	94325..94750	+	426	WP_013831360.1	hypothetical protein	-
PP1Y_RS00685	94799..96049	-	1251	WP_013831361.1	HipA domain-containing protein	-
PP1Y_RS00690	96056..96397	-	342	WP_013831362.1	helix-turn-helix domain-containing protein	-
PP1Y_RS00700	96532..96852	-	321	WP_041558022.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..192103	192103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-64)

Sequences

Toxin

Protein

• Length: 422 a.a.
• Molecular weight: 44399.79 Da
• Isoelectric Point: 5.7788

>T25311 WP_013831356.1 NC_015579:c92156-90891 [Novosphingobium sp. PP1Y]
MAADILDRLVVWSDRRIAGELAIDQGGAMHFAYAEDWLADCGAPALSHALPKQAGAFGDALCKAVFGGLLPEEGQRTAIA
RALGVSPDNPFRLLAALGGDVAGALAFLPPGEQPPPAPTESRPEALDDAALADLLQRLPRAPMLAGEGGARFSLAGAQGK
LPVVLANGRIAVPRIGEPSTHLIKPEPERFAGLAANEAFCLALARAVGLDAATGQWQAVAGKPYLLVSRYDRVERNGQIQ
RLHQEDFAQALGVPSNRKYASEGGPTFRDCFALVRQAATRPAREVLKLADAAIFNLIIGNGDAHAKNFSLLRREDGEVVL
APLYDLVATFIWPELSARLAMRFGPAASLEEVDGDSLIRFAQDSGLTLPFLRRRAAALAGQVEAAIARGIEVPGLADTAL
SGELSQAISDRAGRLVLKARG

Nucleotide

• Length: 1266 bp

>T25311 NC_015579:c92156-90891 [Novosphingobium sp. PP1Y]
ATGGCTGCTGACATTCTCGACCGCCTCGTCGTCTGGTCCGATCGCCGCATCGCGGGCGAACTTGCGATCGATCAAGGCGG
GGCGATGCATTTCGCTTATGCCGAGGACTGGCTGGCGGATTGCGGGGCCCCTGCCCTGTCCCACGCCCTGCCCAAGCAGG
CGGGAGCCTTCGGCGATGCCTTGTGCAAGGCCGTGTTCGGAGGGCTGCTTCCCGAAGAAGGGCAGCGCACGGCGATTGCC
CGTGCGCTGGGCGTTTCACCCGACAACCCGTTCCGGCTGCTGGCGGCGCTGGGCGGCGATGTTGCAGGCGCCCTTGCCTT
CCTGCCCCCGGGGGAGCAACCGCCCCCGGCCCCGACCGAGAGCCGCCCAGAGGCGCTCGATGACGCGGCGCTCGCCGATC
TTCTCCAGCGCTTGCCCCGGGCTCCCATGCTGGCGGGCGAAGGGGGGGCACGCTTCAGCCTTGCCGGGGCGCAGGGCAAG
CTCCCCGTGGTCCTGGCAAACGGGAGGATCGCCGTACCGCGCATCGGCGAACCTTCGACTCACCTGATCAAGCCGGAGCC
CGAACGCTTTGCCGGTCTGGCCGCCAATGAGGCCTTCTGTCTTGCGCTTGCGCGCGCTGTAGGGCTCGATGCAGCGACCG
GGCAGTGGCAGGCAGTTGCCGGGAAGCCCTACCTGCTCGTCTCGCGCTACGACCGGGTTGAAAGGAACGGGCAGATCCAG
CGGCTGCATCAGGAAGATTTCGCGCAGGCGCTGGGCGTGCCGTCCAACCGGAAATACGCCAGCGAGGGCGGACCGACCTT
CCGCGATTGCTTTGCGCTCGTGCGGCAGGCCGCCACGCGCCCGGCGCGGGAGGTTCTCAAGCTGGCCGATGCAGCGATCT
TCAACCTGATCATCGGCAACGGCGACGCCCATGCCAAGAATTTCAGCCTGCTGCGCCGGGAGGATGGGGAAGTCGTCCTT
GCCCCGCTCTACGATCTGGTGGCGACGTTCATCTGGCCGGAACTTTCCGCAAGGCTCGCCATGCGCTTCGGCCCAGCTGC
CAGCCTCGAGGAAGTGGATGGAGACAGCCTGATACGGTTCGCGCAGGACAGCGGCCTCACCCTGCCCTTCCTGCGCCGGC
GCGCCGCGGCTCTGGCTGGACAAGTCGAAGCGGCGATTGCCAGGGGTATCGAGGTTCCCGGTCTGGCAGATACGGCCCTG
TCCGGCGAGCTGTCTCAGGCCATCTCGGACCGGGCCGGCCGACTGGTTCTCAAGGCACGCGGATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8336.64 Da
• Isoelectric Point: 5.0787

>AT25311 WP_013831357.1 NC_015579:c92382-92146 [Novosphingobium sp. PP1Y]
MDTAEIGQIIREERKALGLHQDQLAAASGVGLRFIVELERGKVTAQIGKVIAVLAALGCEVQVLRPGDLRPEERGDGC

Nucleotide

• Length: 237 bp

>AT25311 NC_015579:c92382-92146 [Novosphingobium sp. PP1Y]
ATGGACACGGCCGAAATCGGGCAGATCATTCGCGAGGAACGCAAGGCGCTGGGCCTGCACCAAGATCAGCTGGCGGCAGC
AAGCGGGGTGGGCTTGCGCTTCATCGTCGAGTTGGAGCGAGGCAAGGTCACGGCGCAGATCGGCAAGGTGATCGCGGTCT
TGGCGGCGCTGGGCTGCGAAGTGCAGGTCCTGCGTCCCGGAGATCTCCGGCCGGAGGAGCGCGGCGATGGCTGCTGA

Structures

Toxin

• AlphaFold DB: F6IC73