Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3625969..3626545 Replicon chromosome
Accession NZ_CP102061
Organism Escherichia coli strain C31

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag NP943_RS17645 Protein ID WP_001295743.1
Coordinates 3625969..3626256 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag NP943_RS17650 Protein ID WP_000063148.1
Coordinates 3626243..3626545 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP943_RS17630 (3621629) 3621629..3622933 - 1305 WP_000535012.1 restriction endonuclease subunit S -
NP943_RS17635 (3622923) 3622923..3624542 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
NP943_RS17640 (3624606) 3624606..3625772 - 1167 WP_000800831.1 restriction endonuclease -
NP943_RS17645 (3625969) 3625969..3626256 + 288 WP_001295743.1 BrnT family toxin Toxin
NP943_RS17650 (3626243) 3626243..3626545 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
NP943_RS17655 (3626579) 3626579..3627535 - 957 WP_001295745.1 GTPase -
NP943_RS17660 (3627546) 3627546..3627749 - 204 WP_000467859.1 YbdD/YjiX family protein -
NP943_RS17665 (3627799) 3627799..3629949 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T253090 WP_001295743.1 NZ_CP102061:3625969-3626256 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT253090 WP_000063148.1 NZ_CP102061:3626243-3626545 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References