Detailed information of TA system TA025309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3721205..3721830
• Replicon: chromosome
• Accession: NC_015578
• Organism: Treponema primitia ZAS-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5YKN6
• Locus tag: TREPR_RS16140
• Protein ID: WP_015709418.1
• Coordinates: 3721205..3721600 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: TREPR_RS16145
• Protein ID: WP_041611775.1
• Coordinates: 3721600..3721830 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TREPR_RS16120	3717765..3717989	-	225	WP_015709414.1	NifU family protein	-
TREPR_RS16125	3718180..3719538	+	1359	WP_015709415.1	ATP-dependent 6-phosphofructokinase	-
TREPR_RS16130	3719535..3720398	-	864	WP_015709416.1	hypothetical protein	-
TREPR_RS16135	3720400..3721176	-	777	WP_015709417.1	RNA methyltransferase	-
TREPR_RS16140	3721205..3721600	-	396	WP_015709418.1	type II toxin-antitoxin system VapC family toxin	Toxin
TREPR_RS16145	3721600..3721830	-	231	WP_041611775.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TREPR_RS18705	3722472..3723242	-	771	Protein_3185	endonuclease/exonuclease/phosphatase family protein	-
TREPR_RS16155	3723335..3724156	-	822	WP_015709421.1	cytidylate kinase family protein	-
TREPR_RS16160	3724282..3724866	+	585	WP_015709422.1	NUDIX hydrolase	-
TREPR_RS16165	3724848..3725576	-	729	WP_015709423.1	LysM peptidoglycan-binding domain-containing protein	-
TREPR_RS16170	3725573..3726742	-	1170	WP_015709424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15081.92 Da
• Isoelectric Point: 10.0668

>T25309 WP_015709418.1 NC_015578:c3721600-3721205 [Treponema primitia ZAS-2]
MYMLDTNICIYIIKKKNPNVLKKLKSRWKKGLAISTITLAELEFGNENSMYKEKNQIALIEFLSIIDIKSFDEKATKEYG
IIKKGLKDRQCLIGPLDMLIGAHAKSLGMTLVTNNTKEFERIKNLKVENWV

Nucleotide

• Length: 396 bp

>T25309 NC_015578:c3721600-3721205 [Treponema primitia ZAS-2]
ATGTACATGCTTGATACAAATATATGTATATATATTATCAAGAAGAAAAATCCCAATGTATTGAAAAAATTAAAATCAAG
ATGGAAAAAGGGCCTGGCAATTTCGACAATAACATTGGCTGAATTGGAATTTGGTAATGAAAATAGTATGTATAAAGAAA
AGAATCAAATTGCCTTAATCGAATTTTTGTCAATAATAGATATAAAAAGTTTTGATGAAAAGGCAACGAAAGAATATGGA
ATAATAAAGAAGGGCTTAAAAGACAGGCAATGTTTAATAGGGCCGCTAGACATGTTAATAGGAGCCCATGCAAAATCACT
GGGTATGACATTGGTGACAAATAACACAAAAGAATTTGAAAGGATAAAGAATTTAAAGGTAGAAAATTGGGTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8804.89 Da
• Isoelectric Point: 4.7513

>AT25309 WP_041611775.1 NC_015578:c3721830-3721600 [Treponema primitia ZAS-2]
MQTARLFINGRSQAVRLPKEYQFKGENVYIQKVGDAVMLFPVDKGWETFLHGLNGFSEDFMSEGRYQGADQEREEL

Nucleotide

• Length: 231 bp

>AT25309 NC_015578:c3721830-3721600 [Treponema primitia ZAS-2]
TTGCAAACAGCGCGTTTGTTTATAAATGGCAGGAGCCAAGCTGTAAGACTCCCTAAAGAATATCAGTTTAAAGGTGAAAA
TGTATATATTCAGAAGGTAGGGGACGCTGTCATGCTATTTCCTGTTGATAAGGGATGGGAAACATTTTTACATGGGCTTA
ATGGATTTTCTGAAGATTTTATGAGCGAAGGAAGATATCAGGGCGCAGATCAGGAAAGAGAAGAGTTATAA

Structures

Toxin

• AlphaFold DB: F5YKN6