Detailed information of TA system TA025306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 3121941..3122512
• Replicon: chromosome
• Accession: NC_015578
• Organism: Treponema primitia ZAS-2

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: TREPR_RS13475
• Protein ID: WP_201765784.1
• Coordinates: 3121941..3122270 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: TREPR_RS13480
• Protein ID: WP_041611701.1
• Coordinates: 3122258..3122512 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TREPR_RS13460	3117746..3119455	+	1710	WP_148257475.1	hypothetical protein	-
TREPR_RS13465	3119461..3121299	+	1839	WP_015708875.1	S1C family serine protease	-
TREPR_RS13470	3121306..3121785	-	480	WP_015708876.1	pyridoxamine 5'-phosphate oxidase family protein	-
TREPR_RS13475	3121941..3122270	-	330	WP_201765784.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TREPR_RS13480	3122258..3122512	-	255	WP_041611701.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TREPR_RS13485	3122697..3123356	+	660	WP_174269891.1	outer membrane lipoprotein carrier protein LolA	-
TREPR_RS13490	3123428..3124552	+	1125	WP_015708880.1	helix-turn-helix domain-containing protein	-
TREPR_RS13495	3124588..3126021	+	1434	WP_174269892.1	30S ribosomal protein S12 methylthiotransferase RimO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

• SpoVT-AbrB domain (8-48)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12484.86 Da
• Isoelectric Point: 10.2440

>T25306 WP_201765784.1 NC_015578:c3122270-3121941 [Treponema primitia ZAS-2]
MGTVMEQYDIFLINLDPTIGHEIKKTRPCLLISPNEMNENIATVIIAPMTTKSHKYPTRVKISLNGTKGWVVLDQIRSID
KKRILKKIGKLNAKNIEEVKNIIREMLVE

Nucleotide

• Length: 330 bp

>T25306 NC_015578:c3122270-3121941 [Treponema primitia ZAS-2]
TTGGGAACCGTAATGGAACAGTACGATATTTTTTTGATAAATCTTGATCCGACAATAGGGCATGAAATAAAGAAAACACG
GCCATGTCTGCTTATTTCTCCAAATGAAATGAATGAAAATATTGCAACGGTTATTATTGCGCCAATGACAACAAAATCAC
ATAAATATCCAACCCGGGTAAAGATTAGTTTGAATGGAACTAAAGGATGGGTTGTTTTGGATCAAATACGATCAATCGAT
AAAAAAAGGATACTAAAGAAAATTGGAAAGTTAAATGCAAAAAACATAGAAGAAGTAAAGAATATAATAAGAGAAATGCT
GGTAGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9835.31 Da
• Isoelectric Point: 4.5370

>AT25306 WP_041611701.1 NC_015578:c3122512-3122258 [Treponema primitia ZAS-2]
MIVSVVSIGNSRGIRIPKSIINEFNIEDKIELQIHEDELVLKPISKKPRLGWEKAFKKMNENLDDKLIISNTLEDDVFDW
DWEP

Nucleotide

• Length: 255 bp

>AT25306 NC_015578:c3122512-3122258 [Treponema primitia ZAS-2]
ATGATTGTTTCAGTTGTATCTATTGGCAATTCCAGGGGCATTAGAATTCCCAAAAGTATTATAAATGAATTTAATATTGA
GGATAAAATTGAATTGCAGATACATGAAGATGAATTGGTATTAAAGCCTATATCAAAAAAGCCCCGCTTGGGGTGGGAAA
AGGCGTTTAAAAAAATGAACGAAAATCTTGATGACAAACTCATCATATCAAATACCCTGGAGGATGATGTCTTTGATTGG
GATTGGGAACCGTAA