253021

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1327421..1328337 Replicon chromosome
Accession NZ_CP101937
Organism Bacillus subtilis strain RUB331

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NP435_RS06965 Protein ID WP_003244695.1
Coordinates 1327591..1328337 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NP435_RS06960 Protein ID WP_003232646.1
Coordinates 1327421..1327591 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP435_RS06925 (1324284) 1324284..1324613 + 330 WP_003232660.1 XkdW family protein -
NP435_RS06930 (1324610) 1324610..1324774 + 165 WP_003232658.1 XkdX family protein -
NP435_RS06935 (1324818) 1324818..1325657 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
NP435_RS06940 (1325710) 1325710..1325979 + 270 WP_003232655.1 hemolysin XhlA family protein -
NP435_RS06945 (1325992) 1325992..1326255 + 264 WP_003232653.1 phage holin -
NP435_RS06950 (1326268) 1326268..1327161 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
NP435_RS06955 (1327198) 1327198..1327335 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NP435_RS06960 (1327421) 1327421..1327591 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NP435_RS06965 (1327591) 1327591..1328337 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NP435_RS06970 (1328447) 1328447..1329448 - 1002 WP_003232642.1 inorganic phosphate transporter -
NP435_RS06975 (1329461) 1329461..1330078 - 618 WP_003218470.1 DUF47 domain-containing protein -
NP435_RS06980 (1330354) 1330354..1331670 - 1317 WP_003244819.1 serine/threonine exchanger -
NP435_RS06985 (1332059) 1332059..1333009 + 951 WP_003245086.1 ring-cleaving dioxygenase -
NP435_RS06990 (1333110) 1333110..1333256 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T253021 WP_003244695.1 NZ_CP101937:c1328337-1327591 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT253021 WP_003232646.1 NZ_CP101937:c1327591-1327421 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References