253020

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518243..518879 Replicon chromosome
Accession NZ_CP101937
Organism Bacillus subtilis strain RUB331

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NP435_RS02640 Protein ID WP_003156187.1
Coordinates 518529..518879 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NP435_RS02635 Protein ID WP_003225183.1
Coordinates 518243..518524 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP435_RS02615 (514602) 514602..515201 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
NP435_RS02620 (515296) 515296..515661 + 366 WP_003234281.1 holo-ACP synthase -
NP435_RS02625 (515827) 515827..516843 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NP435_RS02630 (516958) 516958..518127 + 1170 WP_003234284.1 alanine racemase -
NP435_RS02635 (518243) 518243..518524 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NP435_RS02640 (518529) 518529..518879 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NP435_RS02645 (518994) 518994..519818 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NP435_RS02650 (519823) 519823..520188 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NP435_RS02655 (520192) 520192..520593 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NP435_RS02660 (520605) 520605..521612 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NP435_RS02665 (521674) 521674..522003 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NP435_RS02670 (522000) 522000..522482 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NP435_RS02675 (522448) 522448..523236 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NP435_RS02680 (523236) 523236..523835 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T253020 WP_003156187.1 NZ_CP101937:518529-518879 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT253020 WP_003225183.1 NZ_CP101937:518243-518524 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References