253017

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1366100..1367016 Replicon chromosome
Accession NZ_CP101933
Organism Bacillus subtilis subsp. natto strain Bacillus subtilis 'natto'

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NP437_RS07250 Protein ID WP_003244695.1
Coordinates 1366270..1367016 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NP437_RS07245 Protein ID WP_003232646.1
Coordinates 1366100..1366270 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP437_RS07210 (NP437_07210) 1362959..1363288 + 330 WP_014479562.1 XkdW family protein -
NP437_RS07215 (NP437_07215) 1363285..1363449 + 165 WP_014479563.1 XkdX family protein -
NP437_RS07220 (NP437_07220) 1363496..1364335 + 840 WP_014479564.1 phage-like element PBSX protein XepA -
NP437_RS07225 (NP437_07225) 1364388..1364657 + 270 WP_014479565.1 hemolysin XhlA family protein -
NP437_RS07230 (NP437_07230) 1364670..1364933 + 264 WP_014479566.1 phage holin -
NP437_RS07235 (NP437_07235) 1364946..1365839 + 894 WP_014479567.1 N-acetylmuramoyl-L-alanine amidase -
NP437_RS07240 (NP437_07240) 1365877..1366014 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NP437_RS07245 (NP437_07245) 1366100..1366270 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NP437_RS07250 (NP437_07250) 1366270..1367016 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NP437_RS07255 (NP437_07255) 1367126..1368127 - 1002 WP_014479569.1 inorganic phosphate transporter -
NP437_RS07260 (NP437_07260) 1368140..1368757 - 618 WP_003218470.1 DUF47 domain-containing protein -
NP437_RS07265 (NP437_07265) 1369033..1370349 - 1317 WP_014479570.1 serine/threonine exchanger -
NP437_RS07270 (NP437_07270) 1370738..1371688 + 951 WP_014479571.1 ring-cleaving dioxygenase -
NP437_RS07275 (NP437_07275) 1371797..1371892 + 96 Protein_1370 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T253017 WP_003244695.1 NZ_CP101933:c1367016-1366270 [Bacillus subtilis subsp. natto]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT253017 WP_003232646.1 NZ_CP101933:c1366270-1366100 [Bacillus subtilis subsp. natto]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References