Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 511422..512058 Replicon chromosome
Accession NZ_CP101932
Organism Bacillus subtilis subsp. natto strain BGSC 27E1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NP436_RS02625 Protein ID WP_003156187.1
Coordinates 511708..512058 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NP436_RS02620 Protein ID WP_003225183.1
Coordinates 511422..511703 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP436_RS02600 (NP436_02600) 507781..508380 - 600 WP_014478896.1 rhomboid family intramembrane serine protease -
NP436_RS02605 (NP436_02605) 508475..508840 + 366 WP_014478897.1 holo-ACP synthase -
NP436_RS02610 (NP436_02610) 509006..510022 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NP436_RS02615 (NP436_02615) 510137..511306 + 1170 WP_014478898.1 alanine racemase -
NP436_RS02620 (NP436_02620) 511422..511703 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NP436_RS02625 (NP436_02625) 511708..512058 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NP436_RS02630 (NP436_02630) 512174..512998 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NP436_RS02635 (NP436_02635) 513003..513368 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NP436_RS02640 (NP436_02640) 513372..513773 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
NP436_RS02645 (NP436_02645) 513785..514792 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
NP436_RS02650 (NP436_02650) 514861..515190 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NP436_RS02655 (NP436_02655) 515187..515669 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NP436_RS02660 (NP436_02660) 515635..516423 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NP436_RS02665 (NP436_02665) 516423..517022 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T253014 WP_003156187.1 NZ_CP101932:511708-512058 [Bacillus subtilis subsp. natto]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT253014 WP_003225183.1 NZ_CP101932:511422-511703 [Bacillus subtilis subsp. natto]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References