Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 525546..526182 Replicon chromosome
Accession NZ_CP101930
Organism Bacillus subtilis strain BGSC 10A5

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NP433_RS02750 Protein ID WP_003156187.1
Coordinates 525832..526182 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NP433_RS02745 Protein ID WP_003225183.1
Coordinates 525546..525827 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP433_RS02725 (NP433_02725) 521905..522504 - 600 WP_123372836.1 rhomboid family intramembrane serine protease -
NP433_RS02730 (NP433_02730) 522599..522964 + 366 WP_256683226.1 holo-ACP synthase -
NP433_RS02735 (NP433_02735) 523130..524146 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NP433_RS02740 (NP433_02740) 524261..525430 + 1170 WP_017697002.1 alanine racemase -
NP433_RS02745 (NP433_02745) 525546..525827 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NP433_RS02750 (NP433_02750) 525832..526182 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NP433_RS02755 (NP433_02755) 526297..527121 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NP433_RS02760 (NP433_02760) 527126..527491 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NP433_RS02765 (NP433_02765) 527495..527896 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NP433_RS02770 (NP433_02770) 527908..528915 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NP433_RS02775 (NP433_02775) 528977..529306 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NP433_RS02780 (NP433_02780) 529303..529785 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NP433_RS02785 (NP433_02785) 529751..530539 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NP433_RS02790 (NP433_02790) 530539..531138 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T253010 WP_003156187.1 NZ_CP101930:525832-526182 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT253010 WP_003225183.1 NZ_CP101930:525546-525827 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References