Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4608043..4608540 Replicon chromosome
Accession NZ_CP101925
Organism Escherichia coli strain Seattle 1946

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NP429_RS22290 Protein ID WP_001173343.1
Coordinates 4608043..4608216 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag NP429_RS22295 Protein ID WP_001312331.1
Coordinates 4608244..4608540 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP429_RS22280 (4604713) 4604713..4606170 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
NP429_RS22285 (4606407) 4606407..4607924 + 1518 WP_000003806.1 lysine--tRNA ligase -
NP429_RS22290 (4608043) 4608043..4608216 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NP429_RS22295 (4608244) 4608244..4608540 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NP429_RS22300 (4608769) 4608769..4609041 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NP429_RS22305 (4609053) 4609053..4609283 - 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
NP429_RS22310 (4609464) 4609464..4611095 + 1632 WP_001216466.1 sensor histidine kinase -
NP429_RS22315 (4611092) 4611092..4611811 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T253006 WP_001173343.1 NZ_CP101925:c4608216-4608043 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT253006 WP_001312331.1 NZ_CP101925:c4608540-4608244 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References